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| ==Crystal structure of CRISPR-associated protein== | | ==Crystal structure of CRISPR-associated protein== |
| <StructureSection load='4q2c' size='340' side='right'caption='[[4q2c]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='4q2c' size='340' side='right'caption='[[4q2c]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4q2c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2C FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2C FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2c OCA], [https://pdbe.org/4q2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2c RCSB], [https://www.ebi.ac.uk/pdbsum/4q2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2c ProSAT]</span></td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4q2d|4q2d]]</div></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2c OCA], [https://pdbe.org/4q2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2c RCSB], [https://www.ebi.ac.uk/pdbsum/4q2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2c ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Mobile genetic elements in bacteria are neutralized by a system based on clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins. Type I CRISPR-Cas systems use a "Cascade" ribonucleoprotein complex to guide RNA specifically to complementary sequence in invader double-stranded DNA (dsDNA), a process called "interference." After target recognition by Cascade, formation of an R-loop triggers recruitment of a Cas3 nuclease-helicase, completing the interference process by destroying the invader dsDNA. To elucidate the molecular mechanism of CRISPR interference, we analyzed crystal structures of Cas3 from the bacterium Thermobaculum terrenum, with and without a bound ATP analog. The structures reveal a histidine-aspartate (HD)-type nuclease domain fused to superfamily-2 (SF2) helicase domains and a distinct C-terminal domain. Binding of ATP analog at the interface of the SF2 helicase RecA-like domains rearranges a motif V with implications for the enzyme mechanism. The HD-nucleolytic site contains two metal ions that are positioned at the end of a proposed nucleic acid-binding tunnel running through the SF2 helicase structure. This structural alignment suggests a mechanism for 3' to 5' nucleolytic processing of the displaced strand of invader DNA that is coordinated with ATP-dependent 3' to 5' translocation of Cas3 along DNA. In agreement with biochemical studies, the presented Cas3 structures reveal important mechanistic details on the neutralization of genetic invaders by type I CRISPR-Cas systems.
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| Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3.,Gong B, Shin M, Sun J, Jung CH, Bolt EL, van der Oost J, Kim JS Proc Natl Acad Sci U S A. 2014 Nov 3. pii: 201410806. PMID:25368186<ref>PMID:25368186</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4q2c" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Endonuclease 3D structures|Endonuclease 3D structures]] | | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| *[[Helicase 3D structures|Helicase 3D structures]] | | *[[Helicase 3D structures|Helicase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Gong, B]] | | [[Category: Gong B]] |
| [[Category: Kim, J S]] | | [[Category: Kim J-S]] |
| [[Category: Oost, J van der]]
| | [[Category: Shin M]] |
| [[Category: Shin, M]] | | [[Category: Sun J]] |
| [[Category: Sun, J]] | | [[Category: Van der Oost J]] |
| [[Category: Hd nuclease]] | |
| [[Category: Hydrolase]]
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| [[Category: Reca]]
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