7rom: Difference between revisions
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==Crystal structure of Saccharomyces cerevisiae NADH-cytochrome b5 reductase 1 (Cbr1) fragment (residues 28-284) bound to FAD== | ==Crystal structure of Saccharomyces cerevisiae NADH-cytochrome b5 reductase 1 (Cbr1) fragment (residues 28-284) bound to FAD== | ||
<StructureSection load='7rom' size='340' side='right'caption='[[7rom]]' scene=''> | <StructureSection load='7rom' size='340' side='right'caption='[[7rom]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ROM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ROM FirstGlance]. <br> | <table><tr><td colspan='2'>[[7rom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ROM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ROM FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rom OCA], [https://pdbe.org/7rom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rom RCSB], [https://www.ebi.ac.uk/pdbsum/7rom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rom ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rom OCA], [https://pdbe.org/7rom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rom RCSB], [https://www.ebi.ac.uk/pdbsum/7rom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rom ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/NCB5R_YEAST NCB5R_YEAST] NADH-dependent reductase for KTI11/DPH3 and cytochrome b5 (PubMed:14930, PubMed:10622712, PubMed:27694803, PubMed:31463593, PubMed:34154323). Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed:31463593, PubMed:34154323, PubMed:27694803). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising KTI11/DPH3 and a NADH-dependent reductase, predominantly CBR1 (PubMed:31463593, PubMed:34154323). By reducing KTI11/DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex (PubMed:27694803). The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes (PubMed:10622712). Plays a role in bud morphology (PubMed:17895367).<ref>PMID:10622712</ref> <ref>PMID:14930</ref> <ref>PMID:17895367</ref> <ref>PMID:27694803</ref> <ref>PMID:31463593</ref> <ref>PMID:34154323</ref> | |||
==See Also== | |||
*[[NADH-cytochrome b5 reductase|NADH-cytochrome b5 reductase]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae]] | |||
[[Category: Fenwick MK]] | [[Category: Fenwick MK]] | ||
[[Category: Lin H]] | [[Category: Lin H]] | ||
[[Category: Zhang Y]] | [[Category: Zhang Y]] | ||