8a5d: Difference between revisions
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==Structure of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of Chaetomium thermophilum INO80== | |||
<StructureSection load='8a5d' size='340' side='right'caption='[[8a5d]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8a5d]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A5D FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a5d OCA], [https://pdbe.org/8a5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a5d RCSB], [https://www.ebi.ac.uk/pdbsum/8a5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a5d ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G0S519_CHATD G0S519_CHATD] Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex.[RuleBase:RU364121] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The nucleosomal landscape of chromatin depends on the concerted action of chromatin remodelers. The INO80 remodeler specifically places nucleosomes at the boundary of gene regulatory elements, which is proposed to be the result of an ATP-dependent nucleosome sliding activity that is regulated by extranucleosomal DNA features. Here, we use cryo-electron microscopy and functional assays to reveal how INO80 binds and is regulated by extranucleosomal DNA. Structures of the regulatory A-module bound to DNA clarify the mechanism of linker DNA binding. The A-module is connected to the motor unit via an HSA/post-HSA lever element to chemomechanically couple the motor and linker DNA sensing. Two notable sites of curved DNA recognition by coordinated action of the four actin/actin-related proteins and the motor suggest how sliding by INO80 can be regulated by extranucleosomal DNA features. Last, the structures clarify the recruitment of YY1/Ies4 subunits and reveal deep architectural similarities between the regulatory modules of INO80 and SWI/SNF complexes. | |||
Structural mechanism of extranucleosomal DNA readout by the INO80 complex.,Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP Sci Adv. 2022 Dec 9;8(49):eadd3189. doi: 10.1126/sciadv.add3189. Epub 2022 Dec 9. PMID:36490333<ref>PMID:36490333</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8a5d" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Jung | <references/> | ||
[[Category: Kostrewa | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Chaetomium thermophilum]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Eustermann S]] | ||
[[Category: Hopfner KP]] | |||
[[Category: Jung J]] | |||
[[Category: Kostrewa D]] | |||
[[Category: Kunert F]] | |||
[[Category: Metzner FJ]] | |||
[[Category: Schall K]] | |||
[[Category: Woike S]] | |||