7xso: Difference between revisions

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-'''Unreleased structure'''
-The entry 7xso is ON HOLD  until Paper Publication
+==Structure of the type III-E CRISPR-Cas effector gRAMP==
+<StructureSection load='7xso' size='340' side='right'caption='[[7xso]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7xso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSO FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xso OCA], [https://pdbe.org/7xso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xso RCSB], [https://www.ebi.ac.uk/pdbsum/7xso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xso ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In the type III-E CRISPR-Cas system, a Cas effector (gRAMP) is associated with a TPR-CHAT to form Craspase (CRISPR-guided caspase). However, both the structural features of gRAMP and the immunity mechanism remain unknown for this system. Here, we report structures of gRAMP-crRNA and gRAMP:cRNA:target RNA as well as structures of Craspase and Craspase complexed with cognate target RNA (CTR) or non-cognate target RNA (NTR). Importantly, the 3' anti-tag region of NTR and CTR binds at two distinct channels in Craspase, and CTR with a non-complementary 3' anti-tag induces a marked conformational change of the TPR-CHAT, which allosterically activates its protease activity to cleave an ancillary protein Csx30. This cleavage then triggers an abortive infection as the antiviral strategy of the type III-E system. Together, our study provides crucial insights into both the catalytic mechanism of the gRAMP and the immunity mechanism of the type III-E system.
-Authors:
+Target RNA activates the protease activity of Craspase to confer antiviral defense.,Liu X, Zhang L, Wang H, Xiu Y, Huang L, Gao Z, Li N, Li F, Xiong W, Gao T, Zhang Y, Yang M, Feng Y Mol Cell. 2022 Oct 21. pii: S1097-2765(22)00964-9. doi:, 10.1016/j.molcel.2022.10.007. PMID:36306795<ref>PMID:36306795</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7xso" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Candidatus Scalindua brodae]]
+[[Category: Large Structures]]
+[[Category: Feng Y]]
+[[Category: Zhang L]]