3p03: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
Line 3: Line 3:
<StructureSection load='3p03' size='340' side='right'caption='[[3p03]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
<StructureSection load='3p03' size='340' side='right'caption='[[3p03]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3p03]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P03 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3p03]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P03 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">betP, Cgl0892, cg1016 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 "Micrococcus glutamicus" Kinoshita et al. 1958])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p03 OCA], [https://pdbe.org/3p03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p03 RCSB], [https://www.ebi.ac.uk/pdbsum/3p03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p03 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p03 OCA], [https://pdbe.org/3p03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p03 RCSB], [https://www.ebi.ac.uk/pdbsum/3p03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p03 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/BETP_CORGL BETP_CORGL]] High-affinity uptake of glycine betaine (By similarity).  
[https://www.uniprot.org/uniprot/BETP_CORGL BETP_CORGL] High-affinity uptake of glycine betaine (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BetP is an Na(+)-coupled betaine-specific transporter of the betaine-choline-carnitine (BCC) transporter family involved in the response to hyperosmotic stress. The crystal structure of BetP revealed an overall fold of two inverted structurally related repeats (LeuT-fold) that BetP shares with other sequence-unrelated Na(+)-coupled symporters. Numerous structures of LeuT-fold transporters in distinct conformational states have contributed substantially to our understanding of the alternating access mechanism of transport. Nevertheless, coupling of substrate and co-transported ion fluxes has not been structurally corroborated to the same extent. We converted BetP by a single-point mutation-glycine to aspartate-into an H(+)-coupled choline-specific transporter and solved the crystal structure of this mutant in complex with choline. The structure of BetP-G153D demonstrates a new inward-facing open conformation for BetP. Choline binding to a location close to the second, low-affinity sodium-binding site (Na2) of LeuT-fold transporters is facilitated by the introduced aspartate. Our data confirm the importance of a cation-binding site in BetP, playing a key role in a proposed molecular mechanism of Na(+) and H(+) coupling in BCC transporters.
 
Substrate specificity and ion coupling in the Na(+)/betaine symporter BetP.,Perez C, Koshy C, Ressl S, Nicklisch S, Kramer R, Ziegler C EMBO J. 2011 Mar 1. PMID:21364531<ref>PMID:21364531</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3p03" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Symporter 3D structures|Symporter 3D structures]]
*[[Symporter 3D structures|Symporter 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Micrococcus glutamicus kinoshita et al. 1958]]
[[Category: Corynebacterium glutamicum]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Perez, C]]
[[Category: Perez C]]
[[Category: Ressl, S]]
[[Category: Ressl S]]
[[Category: Ziegler, Z]]
[[Category: Ziegler Z]]
[[Category: Secondary transporter]]
[[Category: Transport protein]]

Latest revision as of 13:37, 21 February 2024

Crystal structure of BetP-G153D with choline boundCrystal structure of BetP-G153D with choline bound

Structural highlights

3p03 is a 3 chain structure with sequence from Corynebacterium glutamicum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.35Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BETP_CORGL High-affinity uptake of glycine betaine (By similarity).

See Also

3p03, resolution 3.35Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3p03&oldid=4067080"