1h38: Difference between revisions

Revision as of 06:33, 1 July 2008

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File:1h38.png

Template:STRUCTURE 1h38

STRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTIONSTRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTION

Template:ABSTRACT PUBMED 12422209

About this StructureAbout this Structure

1H38 is a Protein complex structure of sequences from Enterobacteria phage t7. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution., Tahirov TH, Temiakov D, Anikin M, Patlan V, McAllister WT, Vassylyev DG, Yokoyama S, Nature. 2002 Nov 7;420(6911):43-50. Epub 2002 Oct 9. PMID:12422209

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-'''STRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTION'''
+===STRUCTURE OF A T7 RNA POLYMERASE ELONGATION COMPLEX AT 2.9A RESOLUTION===
-==Overview==
+<!--
-The single-subunit bacteriophage T7 RNA polymerase carries out the transcription cycle in an identical manner to that of bacterial and eukaryotic multisubunit enzymes. Here we report the crystal structure of a T7 RNA polymerase elongation complex, which shows that incorporation of an 8-base-pair RNA-DNA hybrid into the active site of the enzyme induces a marked rearrangement of the amino-terminal domain. This rearrangement involves alternative folding of about 130 residues and a marked reorientation (about 130 degrees rotation) of a stable core subdomain, resulting in a structure that provides elements required for stable transcription elongation. A wide opening on the enzyme surface that is probably an RNA exit pathway is formed, and the RNA-DNA hybrid is completely buried in a newly formed, deep protein cavity. Binding of 10 base pairs of downstream DNA is stabilized mostly by long-distance electrostatic interactions. The structure implies plausible mechanisms for the various phases of the transcription cycle, and reveals important structural similarities with the multisubunit RNA polymerases.
+The line below this paragraph, {{ABSTRACT_PUBMED_12422209}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12422209 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_12422209}}
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 [[Category: Structural genomic]]
 [[Category: T7 rna polymerase]]
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