7u05: Difference between revisions
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==== | ==Structure of the yeast TRAPPII-Rab11/Ypt32 complex in the closed/closed state (composite structure)== | ||
<StructureSection load='7u05' size='340' side='right'caption='[[7u05]]' scene=''> | <StructureSection load='7u05' size='340' side='right'caption='[[7u05]], [[Resolution|resolution]] 3.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7u05]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U05 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u05 OCA], [https://pdbe.org/7u05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u05 RCSB], [https://www.ebi.ac.uk/pdbsum/7u05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u05 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u05 OCA], [https://pdbe.org/7u05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u05 RCSB], [https://www.ebi.ac.uk/pdbsum/7u05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u05 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Rab1 and Rab11 are essential regulators of the eukaryotic secretory and endocytic recycling pathways. The transport protein particle (TRAPP) complexes activate these guanosine triphosphatases via nucleotide exchange using a shared set of core subunits. The basal specificity of the TRAPP core is toward Rab1, yet the TRAPPII complex is specific for Rab11. A steric gating mechanism has been proposed to explain TRAPPII counterselection against Rab1. Here, we present cryo-electron microscopy structures of the 22-subunit TRAPPII complex from budding yeast, including a TRAPPII-Rab11 nucleotide exchange intermediate. The Trs130 subunit provides a "leg" that positions the active site distal to the membrane surface, and this leg is required for steric gating. The related TRAPPIII complex is unable to activate Rab11 because of a repulsive interaction, which TRAPPII surmounts using the Trs120 subunit as a "lid" to enclose the active site. TRAPPII also adopts an open conformation enabling Rab11 to access and exit from the active site chamber. | |||
Structure of a TRAPPII-Rab11 activation intermediate reveals GTPase substrate selection mechanisms.,Bagde SR, Fromme JC Sci Adv. 2022 May 13;8(19):eabn7446. doi: 10.1126/sciadv.abn7446. Epub 2022 May , 13. PMID:35559680<ref>PMID:35559680</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7u05" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Bagde SR]] | |||
[[Category: Fromme JC]] | |||