7vyq: Difference between revisions
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==Oligomeric interactions maintain active-site structure in a non-cooperative enzyme family== | ==Oligomeric interactions maintain active-site structure in a non-cooperative enzyme family== | ||
<StructureSection load='7vyq' size='340' side='right'caption='[[7vyq]]' scene=''> | <StructureSection load='7vyq' size='340' side='right'caption='[[7vyq]], [[Resolution|resolution]] 3.13Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VYQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[7vyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VYQ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vyq OCA], [https://pdbe.org/7vyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vyq RCSB], [https://www.ebi.ac.uk/pdbsum/7vyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vyq ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=83I:ethyl+4-chloranyl-3-oxidanylidene-butanoate'>83I</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vyq OCA], [https://pdbe.org/7vyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vyq RCSB], [https://www.ebi.ac.uk/pdbsum/7vyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vyq ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The evolutionary benefit accounting for widespread conservation of oligomeric structures in proteins lacking evidence of intersubunit cooperativity remains unclear. Here, crystal and cryo-EM structures, and enzymological data, demonstrate that a conserved tetramer interface maintains the active-site structure in one such class of proteins, the short-chain dehydrogenase/reductase (SDR) superfamily. Phylogenetic comparisons support a significantly longer polypeptide being required to maintain an equivalent active-site structure in the context of a single subunit. Oligomerization therefore enhances evolutionary fitness by reducing the metabolic cost of enzyme biosynthesis. The large surface area of the structure-stabilizing oligomeric interface yields a synergistic gain in fitness by increasing tolerance to activity-enhancing yet destabilizing mutations. We demonstrate that two paralogous SDR superfamily enzymes with different specificities can form mixed heterotetramers that combine their individual enzymological properties. This suggests that oligomerization can also diversify the functions generated by a given metabolic investment, enhancing the fitness advantage provided by this architectural strategy. | |||
Oligomeric interactions maintain active-site structure in a noncooperative enzyme family.,Li Y, Zhang R, Wang C, Forouhar F, Clarke OB, Vorobiev S, Singh S, Montelione GT, Szyperski T, Xu Y, Hunt JF EMBO J. 2022 Jul 8:e108368. doi: 10.15252/embj.2021108368. PMID:35801308<ref>PMID:35801308</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7vyq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Clarke O]] | [[Category: Clarke, O]] | ||
[[Category: Forouhar F]] | [[Category: Forouhar, F]] | ||
[[Category: Hunt | [[Category: Hunt, J F]] | ||
[[Category: Li | [[Category: Li, Y H]] | ||
[[Category: Montelione | [[Category: Montelione, G T]] | ||
[[Category: Singh S]] | [[Category: Singh, S]] | ||
[[Category: Szyperski T]] | [[Category: Szyperski, T]] | ||
[[Category: Vorobiev S]] | [[Category: Vorobiev, S]] | ||
[[Category: Wang C]] | [[Category: Wang, C]] | ||
[[Category: Xu Y]] | [[Category: Xu, Y]] | ||
[[Category: Zhang | [[Category: Zhang, R Z]] | ||
[[Category: Oxidoreductase]] | |||
[[Category: Rossmann fold]] | |||
[[Category: Tag-free]] | |||
[[Category: Tetramer]] | |||
[[Category: Wild type with nadph]] | |||