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==Crystal structure of Wolbachia cytoplasmic incompatibility factor CidA from wMel== | ==Crystal structure of Wolbachia cytoplasmic incompatibility factor CidA from wMel== | ||
<StructureSection load='7fit' size='340' side='right'caption='[[7fit]]' scene=''> | <StructureSection load='7fit' size='340' side='right'caption='[[7fit]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FIT FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FIT FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fit OCA], [https://pdbe.org/7fit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fit RCSB], [https://www.ebi.ac.uk/pdbsum/7fit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fit ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fit OCA], [https://pdbe.org/7fit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fit RCSB], [https://www.ebi.ac.uk/pdbsum/7fit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fit ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA(wMel) and CidB(wMel). Substituting CidA(wMel) interface residues with those from CidA(wPip) (from strain wPip) enables the mutant protein to bind CidB(wPip) and rescue CidB(wPip)-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA(wPip) and CidB(wPip) proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains. | |||
Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.,Wang H, Xiao Y, Chen X, Zhang M, Sun G, Wang F, Wang L, Zhang H, Zhang X, Yang X, Li W, Wei Y, Yao D, Zhang B, Li J, Cui W, Wang F, Chen C, Shen W, Su D, Bai F, Huang J, Ye S, Zhang L, Ji X, Wang W, Wang Z, Hochstrasser M, Yang H Nat Commun. 2022 Mar 25;13(1):1608. doi: 10.1038/s41467-022-29273-w. PMID:35338130<ref>PMID:35338130</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7fit" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 22:34, 29 May 2024
Crystal structure of Wolbachia cytoplasmic incompatibility factor CidA from wMelCrystal structure of Wolbachia cytoplasmic incompatibility factor CidA from wMel
Structural highlights
Publication Abstract from PubMedCytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA(wMel) and CidB(wMel). Substituting CidA(wMel) interface residues with those from CidA(wPip) (from strain wPip) enables the mutant protein to bind CidB(wPip) and rescue CidB(wPip)-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA(wPip) and CidB(wPip) proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains. Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.,Wang H, Xiao Y, Chen X, Zhang M, Sun G, Wang F, Wang L, Zhang H, Zhang X, Yang X, Li W, Wei Y, Yao D, Zhang B, Li J, Cui W, Wang F, Chen C, Shen W, Su D, Bai F, Huang J, Ye S, Zhang L, Ji X, Wang W, Wang Z, Hochstrasser M, Yang H Nat Commun. 2022 Mar 25;13(1):1608. doi: 10.1038/s41467-022-29273-w. PMID:35338130[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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