7fgf: Difference between revisions
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==Cryo-EM structure of CCHFV envelope protein Gc in postfusion conformation== | ==Cryo-EM structure of CCHFV envelope protein Gc in postfusion conformation== | ||
<StructureSection load='7fgf' size='340' side='right'caption='[[7fgf]]' scene=''> | <StructureSection load='7fgf' size='340' side='right'caption='[[7fgf]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FGF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FGF FirstGlance]. <br> | <table><tr><td colspan='2'>[[7fgf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FGF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FGF FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fgf OCA], [https://pdbe.org/7fgf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fgf RCSB], [https://www.ebi.ac.uk/pdbsum/7fgf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fgf ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fgf OCA], [https://pdbe.org/7fgf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fgf RCSB], [https://www.ebi.ac.uk/pdbsum/7fgf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fgf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/GP_CCHFI GP_CCHFI]] Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion (By similarity).<ref>PMID:19088291</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Crimean-Congo hemorrhagic fever virus (CCHFV) is a causative agent of serious hemorrhagic diseases in humans with high mortality rates. CCHFV glycoprotein Gc plays critical roles in mediating virus-host membrane fusion and has been studied extensively as an immunogen. However, the molecular mechanisms involved in membrane fusion and Gc-specific antibody-antigen interactions remain unresolved largely because structural information of this glycoprotein is missing. We designed a trimeric protein including most of the ectodomain region of Gc from the prototype CCHFV strain, IbAr10200, which enabled the cryo-electron microscopy structure to be solved at a resolution of 2.8 A. The structure confirms that CCHFV Gc is a class II fusion protein. Unexpectedly, structural comparisons with other solved Gc trimers in the postfusion conformation revealed that CCHFV Gc adopted hybrid architectural features of the fusion loops from hantaviruses and domain III from phenuiviruses, suggesting a complex evolutionary pathway among these bunyaviruses. Antigenic sites on CCHFV Gc that protective neutralizing antibodies target were mapped onto the CCHFV Gc structure, providing valuable information that improved our understanding of potential neutralization mechanisms of various antibodies. | |||
Cryo-EM structure of glycoprotein C from Crimean-Congo hemorrhagic fever virus.,Li N, Rao G, Li Z, Yin J, Chong T, Tian K, Fu Y, Cao S Virol Sin. 2022 Feb;37(1):127-137. doi: 10.1016/j.virs.2022.01.015. Epub 2022 Jan, 18. PMID:35234630<ref>PMID:35234630</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7fgf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cao S]] | [[Category: Cao, S]] | ||
[[Category: Fu Y]] | [[Category: Fu, Y]] | ||
[[Category: Li N]] | [[Category: Li, N]] | ||
[[Category: Rao G]] | [[Category: Rao, G]] | ||
[[Category: Bunyavirus]] | |||
[[Category: Cchfv]] | |||
[[Category: Envelope protein]] | |||
[[Category: Postfusion]] | |||
[[Category: Viral protein]] | |||