7wr4: Difference between revisions
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==Crystal structure of OspC3-calmodulin-caspase-4 complex== | |||
<StructureSection load='7wr4' size='340' side='right'caption='[[7wr4]], [[Resolution|resolution]] 2.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7wr4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WR4 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wr4 OCA], [https://pdbe.org/7wr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wr4 RCSB], [https://www.ebi.ac.uk/pdbsum/7wr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wr4 ProSAT]</span></td></tr> | |||
[[Category: | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/OSPC3_SHIFL OSPC3_SHIFL] ADP-riboxanase effector that inhibits host cell pyroptosis (PubMed:23684308, PubMed:34409271, PubMed:34671164). Acts by mediating arginine ADP-riboxanation of host CASP4/CASP11, blocking CASP4/CASP11 autoprocessing (PubMed:34671164, PubMed:35338844, PubMed:35568036). This prevents CASP4 activation and ability to recognize and cleave GSDMD, thereby inhibiting LPS-induced pyroptosis (PubMed:34671164). ADP-riboxanation takes place in two steps: OspC3 first catalyzes ADP-ribosylation of target Arg, and then initiates a deamination to remove one N-omega group (PubMed:34671164). Independently of its ADP-riboxanase activity, acts as an inhibitor of calcium signaling by inhibiting host calmodulin, preventing activation of the JAK-STAT signaling pathway in response to interferon-beta (PubMed:35568036). Mechanistically, acts by binding to the apo form of calmodulin, preventing calcium-binding and ability to activate host CaMK2 (CAMKII), which is required to stimulate the JAK-STAT signaling pathway in response to interferon-beta (PubMed:35568036).<ref>PMID:23684308</ref> <ref>PMID:34409271</ref> <ref>PMID:34671164</ref> <ref>PMID:35338844</ref> <ref>PMID:35568036</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Shigella flexneri]] | |||
[[Category: Ding J]] | |||
[[Category: Hou YJ]] | |||
[[Category: Shao F]] | |||
[[Category: Zeng H]] | |||