Purine repressor: Difference between revisions

Latest revision as of 13:30, 8 February 2022

Function

Purine repressor (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides^[1].

Structural highlights

The . Water molecules are shown as red spheres. The DNA binding domain contains a and a hinge helix binding to to the DNA minor groove. ^[2].

Structure of E. coli purine processor complex with DNA and guanine (PDB entry 1wet)

Drag the structure with the mouse to rotate

3D structures of purine repressor3D structures of purine repressor

Updated on 08-February-2022

ReferencesReferences

↑ Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621
↑ Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621

[2] Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422

[1]

[2]

@@ Line 17: / Line 17: @@
 **[[1dbq]] - EcPurR CBD + Mg - NMR<br />
 **[[1jhz]] - EcPurR CBD (mutant) + Mg <br />
-**[[1o57]] - PurR - ''Bacillus subtilis''<br />
+**[[1o57]] - BsPurR - ''Bacillus subtilis''<br />
 *Purine repressor complex with DNA and nucleotide
@@ Line 28: / Line 28: @@
 **[[2puc]], [[2puf]] - EcPurR (mutant) + DNA + guanine <br />
 **[[1zay]] - EcPurR + DNA + hypoxanthine + di-imino-purine<br />
-**[[7rmw]] – PurR + PPGPP – ''Bacillus subtilis''<br />
+**[[7rmw]] – BsPurR + PPGPP<br />
 }}
 == References ==
 <references/>
 [[Category:Topic Page]]