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| {{STRUCTURE_1g83| PDB=1g83 | SCENE= }} | | {{STRUCTURE_1g83| PDB=1g83 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF FYN SH3-SH2'''
| | ===CRYSTAL STRUCTURE OF FYN SH3-SH2=== |
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| ==Overview==
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| The regulatory fragment of Src kinases, comprising Src homology (SH) 3 and SH2 domains, is responsible for controlled repression of kinase activity. We have used a multidisciplinary approach involving crystallography, NMR, and isothermal titration calorimetry to study the regulatory fragment of Fyn (FynSH32) and its interaction with a physiological activator: a fragment of focal adhesion kinase that contains both phosphotyrosine and polyproline motifs. Although flexible, the preferred disposition of SH3 and SH2 domains in FynSH32 resembles the inactive forms of Hck and Src, differing significantly from LckSH32. This difference, which results from variation in the SH3-SH2 linker sequences, will affect the potential of the regulatory fragments to repress kinase activity. This surprising result implies that the mechanism of repression of Src family members may vary, explaining functional distinctions between Fyn and Lck. The interaction between FynSH32 and focal adhesion kinase is restricted to the canonical SH3 and SH2 binding sites and does not affect the dynamic independence of the two domains. Consequently, the interaction shows no enhancement by an avidity effect. Such an interaction may have evolved to gain specificity through an extended recognition site while maintaining rapid dissociation after signaling. | | The line below this paragraph, {{ABSTRACT_PUBMED_11278857}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11278857 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11278857}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Antiparallel beta sheet]] | | [[Category: Antiparallel beta sheet]] |
| [[Category: Beta barrel]] | | [[Category: Beta barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:15:36 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:51:42 2008'' |