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| [[Image:1g7o.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1g7o| PDB=1g7o | SCENE= }} | | {{STRUCTURE_1g7o| PDB=1g7o | SCENE= }} |
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| '''NMR SOLUTION STRUCTURE OF REDUCED E. COLI GLUTAREDOXIN 2'''
| | ===NMR SOLUTION STRUCTURE OF REDUCED E. COLI GLUTAREDOXIN 2=== |
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| ==Overview==
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| Glutaredoxin 2 (Grx2) from Escherichia coli is distinguished from other glutaredoxins by its larger size, low overall sequence identity and lack of electron donor activity with ribonucleotide reductase. However, catalysis of glutathione (GSH)-dependent general disulfide reduction by Grx2 is extremely efficient. The high-resolution solution structure of E. coli Grx2 shows a two-domain protein, with residues 1 to 72 forming a classical "thioredoxin-fold" glutaredoxin domain, connected by an 11 residue linker to the highly helical C-terminal domain, residues 84 to 215. The active site, Cys9-Pro10-Tyr11-Cys12, is buried in the interface between the two domains, but Cys9 is solvent-accessible, consistent with its role in catalysis. The structures reveal the hither to unknown fact that Grx2 is structurally similar to glutathione-S-transferases (GST), although there is no obvious sequence homology. The similarity of these structures gives important insights into the functional significance of a new class of mammalian GST-like proteins, the single-cysteine omega class, which have glutaredoxin oxidoreductase activity rather than GSH-S-transferase conjugating activity. E. coli Grx 2 is structurally and functionally a member of this new expanding family of large glutaredoxins. The primary function of Grx2 as a GST-like glutaredoxin is to catalyze reversible glutathionylation of proteins with GSH in cellular redox regulation including stress responses.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11453697}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11453697 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11453697}} |
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| ==About this Structure== | | ==About this Structure== |
| 1G7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7O OCA]. | | 1G7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7O OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Nmr]] | | [[Category: Nmr]] |
| [[Category: Reduced form of glutaredoxin]] | | [[Category: Reduced form of glutaredoxin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:14:38 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:50:28 2008'' |