FirstGlance/Evaluating Protein Crosslinks: Difference between revisions
Eric Martz (talk | contribs) |
Eric Martz (talk | contribs) |
||
| Line 128: | Line 128: | ||
</td></tr></table> | </td></tr></table> | ||
[[5d12]] has two copies of a kinase domain in its [[asymmetric unit]]. FirstGlance reports a possible thioester crosslink onlyin chain B. The C-S distance is 1.76 Å, quite reasonable. However, the residues are not modeled as a thiester bond, and Jmol draws many spurious "bonds". The sidechain of Asp348 passes through a gap in the all-features electron density. | [[5d12]] has two copies of a kinase domain in its [[asymmetric unit]]. FirstGlance reports a possible thioester crosslink onlyin chain B. The C-S distance is 1.76 Å, quite reasonable. However, the residues are not modeled as a thiester bond, and Jmol draws many spurious "bonds". The sidechain of Asp348 passes through a gap in the all-features electron density (<span style="color:#00a000;font-weight:bold;">green isomesh</span>, above left). The difference electron density map (above right) reveals insufficient density to account for the position of the Asp sidechain (<span style="color:#b00000;font-weight:bold;">dark red isomesh</span>), while suggesting an alternative position for those sidechain atoms (<span style="color:#0000ff;font-weight:bold;">blue isomesh</span>). | ||
====Lys-Cys NOS Genuine Crosslinks==== | ====Lys-Cys NOS Genuine Crosslinks==== | ||