2j4w: Difference between revisions
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<StructureSection load='2j4w' size='340' side='right'caption='[[2j4w]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='2j4w' size='340' side='right'caption='[[2j4w]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2j4w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2j4w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J4W FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4w OCA], [https://pdbe.org/2j4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j4w RCSB], [https://www.ebi.ac.uk/pdbsum/2j4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j4w ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4w OCA], [https://pdbe.org/2j4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j4w RCSB], [https://www.ebi.ac.uk/pdbsum/2j4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j4w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9TY14_PLAVI Q9TY14_PLAVI] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: | [[Category: Plasmodium vivax]] | ||
[[Category: | [[Category: Bentley GA]] | ||
[[Category: | [[Category: Faure G]] | ||
[[Category: | [[Category: Igonet S]] | ||
[[Category: | [[Category: Kocken CHM]] | ||
[[Category: Riottot | [[Category: Riottot MM]] | ||
[[Category: Thomas | [[Category: Thomas AW]] | ||
[[Category: | [[Category: Vulliez-Le Normand B]] | ||
Revision as of 17:34, 13 December 2023
Structure of a Plasmodium vivax apical membrane antigen 1-Fab F8.12.19 complexStructure of a Plasmodium vivax apical membrane antigen 1-Fab F8.12.19 complex
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedApical membrane antigen 1 (AMA1) has an important, but as yet uncharacterised, role in host cell invasion by the malaria parasite, Plasmodium. The protein, which is quite conserved between Plasmodium species, comprises an ectoplasmic region, a single transmembrane segment and a small cytoplasmic domain. The ectoplasmic region, which can induce protective immunity in animal models of human malaria, is a leading vaccine candidate that has entered clinical trials. The monoclonal antibody F8.12.19, raised against the recombinant ectoplasmic region of AMA1 from Plasmodium vivax, cross-reacts with homologues from Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and Plasmodium falciparum, as shown by immunofluorescence assays on mature schizonts. The binding of F8.12.19 to recombinant AMA1 from both P. vivax and P. falciparum was measured by surface plasmon resonance, revealing an apparent affinity constant that is about 100-fold weaker for the cross-reacting antigen when compared to the cognate antigen. Crystal structure analysis of Fab F8.12.19 complexed to AMA1 from P. vivax and P. falciparum shows that the monoclonal antibody recognises a discontinuous epitope located on domain III of the ectoplasmic region, the major component being a loop containing a cystine knot. The structures provide a basis for understanding the cross-reactivity. Antibody contacts are made mainly to main-chain and invariant side-chain atoms of AMA1; contact antigen residues that differ in sequence are located at the periphery of the antigen-binding site and can be accommodated at the interface between the two components of the complex. The implications for AMA1 vaccine development are discussed. Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation.,Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA J Mol Biol. 2007 Mar 9;366(5):1523-37. Epub 2006 Dec 16. PMID:17229439[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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