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| [[Image:1fy4.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1fy4.png|left|200px]] |
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| {{STRUCTURE_1fy4| PDB=1fy4 | SCENE= }} | | {{STRUCTURE_1fy4| PDB=1fy4 | SCENE= }} |
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| '''FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION'''
| | ===FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION=== |
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| ==Overview==
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| The X-ray structure of F. oxysporum trypsin has been determined at atomic resolution, revealing electron density in the binding site which was interpreted as a peptide bound in the sites S1, S2 and S3. The structure, which was initially determined at 1.07 A resolution and 283 K, has an Arg in the S1 specificity pocket. The study was extended to 0.81 A resolution at 100 K using crystals soaked in Arg, Lys and Gln to study in greater detail the binding at the S1 site. The electron density in the binding site was compared between the different structures and analysed in terms of partially occupied and overlapping components of peptide, solvent water and possibly other chemical moieties. Arg-soaked crystals reveal a density more detailed but similar to the original structure, with the Arg side chain visible in the S1 pocket and residual peptide density in the S2 and S3 sites. The density in the active site is complex and not fully interpreted. Lys at high concentrations displaces Arg in the S1 pocket, while some main-chain density remains in sites S2 and S3. Gln has been shown not to bind. The free peptide in the S1-S3 sites binds in a similar way to the binding loop of BPTI or the inhibitory domain of the Alzheimer's beta-protein precursor, with some differences in the S1 site. | | The line below this paragraph, {{ABSTRACT_PUBMED_11134922}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11134922 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11134922}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Wilson, K S.]] | | [[Category: Wilson, K S.]] |
| [[Category: Beta barrel]] | | [[Category: Beta barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:53:46 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:06:58 2008'' |