7es2: Difference between revisions
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==a mutant of glycosyktransferase in complex with UDP and Reb D== | ==a mutant of glycosyktransferase in complex with UDP and Reb D== | ||
<StructureSection load='7es2' size='340' side='right'caption='[[7es2]]' scene=''> | <StructureSection load='7es2' size='340' side='right'caption='[[7es2]], [[Resolution|resolution]] 2.34Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7es2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es2 OCA], [https://pdbe.org/7es2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es2 RCSB], [https://www.ebi.ac.uk/pdbsum/7es2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es2 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JDO:rebaudioside+D'>JDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7erx|7erx]], [[7ery|7ery]], [[7es0|7es0]], [[7es1|7es1]]</div></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es2 OCA], [https://pdbe.org/7es2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es2 RCSB], [https://www.ebi.ac.uk/pdbsum/7es2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, the most desirable steviol glycosides Reb D and Reb M, devoid of unpleasant aftertaste, are naturally produced only in trace amounts due to low levels of specific beta (1-2) glucosylation in Stevia. Here, we report the biochemical and structural characterization of OsUGT91C1, a glycosyltransferase from Oryza sativa, which is efficient at catalyzing beta (1-2) glucosylation. The enzyme's ability to bind steviol glycoside substrate in three modes underlies its flexibility to catalyze beta (1-2) glucosylation in two distinct orientations as well as beta (1-6) glucosylation. Guided by the structural insights, we engineer this enzyme to enhance the desirable beta (1-2) glucosylation, eliminate beta (1-6) glucosylation, and obtain a promising catalyst for the industrial production of naturally rare but palatable steviol glycosides. | |||
Catalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.,Zhang J, Tang M, Chen Y, Ke D, Zhou J, Xu X, Yang W, He J, Dong H, Wei Y, Naismith JH, Lin Y, Zhu X, Cheng W Nat Commun. 2021 Dec 2;12(1):7030. doi: 10.1038/s41467-021-27144-4. PMID:34857750<ref>PMID:34857750</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7es2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Zhu X]] | [[Category: Zhu, X]] | ||
[[Category: H27a mutant]] | |||
[[Category: Rice glycosyltransferase]] | |||
[[Category: Transferase]] | |||