1cx0: Difference between revisions

Revision as of 13:10, 21 February 2008

HEPATITIS DELTA VIRUS RIBOZYME

OverviewOverview

The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.

About this StructureAbout this Structure

1CX0 is a Single protein structure of sequence from Hepatitis delta virus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582

Page seeded by OCA on Thu Feb 21 12:10:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1cx0.gif|left|200px]]<br />
+[[Image:1cx0.gif|left|200px]]<br /><applet load="1cx0" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1cx0" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1cx0, resolution 2.30&Aring;" />
 '''HEPATITIS DELTA VIRUS RIBOZYME'''<br />
 ==Overview==
-The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only, catalytic RNA known to be required for the viability of a human pathogen., We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic, HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the, RNA to bind a small, basic protein without affecting ribozyme activity., The co-crystal structure shows that the compact catalytic core comprises, five helical segments connected as an intricate nested double pseudoknot., The 5'-hydroxyl leaving group resulting from the self-scission reaction is, buried deep within an active-site cleft produced by juxtaposition of the, helices and five strand-crossovers, and is surrounded by biochemically, important backbone and base functional groups in a manner reminiscent of, protein enzymes.
+The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
 ==About this Structure==
-CX0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CX0 OCA].
+CX0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX0 OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Hepatitis delta virus]]
 [[Category: Single protein]]
-[[Category: Amare, A.R.Ferre-D.]]
+[[Category: Amare, A R.Ferre-D.]]
-[[Category: Doudna, J.A.]]
+[[Category: Doudna, J A.]]
 [[Category: Zhou, K.]]
 [[Category: MG]]
 [[Category: nested double pseudoknot rna structure]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:26:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:36 2008''