7e1q: Difference between revisions
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==Crystal structure of dehydrogenase/isomerase FabX from Helicobacter pylori== | ==Crystal structure of dehydrogenase/isomerase FabX from Helicobacter pylori== | ||
<StructureSection load='7e1q' size='340' side='right'caption='[[7e1q]]' scene=''> | <StructureSection load='7e1q' size='340' side='right'caption='[[7e1q]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E1Q FirstGlance]. <br> | <table><tr><td colspan='2'>[[7e1q]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E1Q FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e1q OCA], [https://pdbe.org/7e1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e1q RCSB], [https://www.ebi.ac.uk/pdbsum/7e1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e1q ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e1q OCA], [https://pdbe.org/7e1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e1q RCSB], [https://www.ebi.ac.uk/pdbsum/7e1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e1q ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Unsaturated fatty acids (UFAs) are essential for functional membrane phospholipids in most bacteria. The bifunctional dehydrogenase/isomerase FabX is an essential UFA biosynthesis enzyme in the widespread human pathogen Helicobacter pylori, a bacterium etiologically related to 95% of gastric cancers. Here, we present the crystal structures of FabX alone and in complexes with an octanoyl-acyl carrier protein (ACP) substrate or with holo-ACP. FabX belongs to the nitronate monooxygenase (NMO) flavoprotein family but contains an atypical [4Fe-4S] cluster absent in all other family members characterized to date. FabX binds ACP via its positively charged alpha7 helix that interacts with the negatively charged alpha2 and alpha3 helices of ACP. We demonstrate that the [4Fe-4S] cluster potentiates FMN oxidation during dehydrogenase catalysis, generating superoxide from an oxygen molecule that is locked in an oxyanion hole between the FMN and the active site residue His182. Both the [4Fe-4S] and FMN cofactors are essential for UFA synthesis, and the superoxide is subsequently excreted by H. pylori as a major resource of peroxide which may contribute to its pathogenic function in the corrosion of gastric mucosa. | |||
Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis.,Zhou J, Zhang L, Zeng L, Yu L, Duan Y, Shen S, Hu J, Zhang P, Song W, Ruan X, Jiang J, Zhang Y, Zhou L, Jia J, Hang X, Tian C, Lin H, Chen HZ, Cronan JE, Bi H, Zhang L Nat Commun. 2021 Nov 26;12(1):6932. doi: 10.1038/s41467-021-27148-0. PMID:34836944<ref>PMID:34836944</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7e1q" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Zhang L]] | [[Category: Zhang, L]] | ||
[[Category: Zhou | [[Category: Zhou, J S]] | ||
[[Category: 4fe-4s cluster]] | |||
[[Category: Flavoprotein]] | |||
[[Category: Fmn]] | |||
[[Category: Unsaturated fatty acid]] | |||