7f1k: Difference between revisions
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==Designed enzyme RA61 M48K/I72D mutant: form IV== | ==Designed enzyme RA61 M48K/I72D mutant: form IV== | ||
<StructureSection load='7f1k' size='340' side='right'caption='[[7f1k]]' scene=''> | <StructureSection load='7f1k' size='340' side='right'caption='[[7f1k]], [[Resolution|resolution]] 1.05Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F1K FirstGlance]. <br> | <table><tr><td colspan='2'>[[7f1k]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F1K FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f1k OCA], [https://pdbe.org/7f1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f1k RCSB], [https://www.ebi.ac.uk/pdbsum/7f1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f1k ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f1k OCA], [https://pdbe.org/7f1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f1k RCSB], [https://www.ebi.ac.uk/pdbsum/7f1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f1k ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Natural aldolase enzymes and created retro-aldolase protein catalysts often catalyze both aldol and retro-aldol reactions depending on the concentrations of the reactants and the products. Here, we report that the directionality of protein catalysts can be altered by replacing one amino acid. The protein catalyst derived from a scaffold of a previously reported retro-aldolase catalyst, catalyzed aldol reactions more efficiently than the previously reported retro-aldolase catalyst. The retro-aldolase catalyst efficiently catalyzed the retro-aldol reaction but was less efficient in catalyzing the aldol reaction. The results indicate that protein catalysts with varying levels of directionality in usually reversibly catalyzed aldol and retro-aldol reactions can be generated from the same protein scaffold. | |||
Varying the Directionality of Protein Catalysts for Aldol and Retro-Aldol Reactions.,Fujioka T, Numoto N, Akama H, Shilpa K, Oka M, Roy PK, Krishna Y, Ito N, Baker D, Oda M, Tanaka F Chembiochem. 2021 Oct 26. doi: 10.1002/cbic.202100435. PMID:34698422<ref>PMID:34698422</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7f1k" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fujioka T]] | [[Category: Fujioka, T]] | ||
[[Category: Ito N]] | [[Category: Ito, N]] | ||
[[Category: Numoto N]] | [[Category: Numoto, N]] | ||
[[Category: Oda M]] | [[Category: Oda, M]] | ||
[[Category: Oka M]] | [[Category: Oka, M]] | ||
[[Category: Tanaka F]] | [[Category: Tanaka, F]] | ||
[[Category: Aldol reaction]] | |||
[[Category: De novo protein]] | |||
[[Category: Mutation]] | |||
[[Category: Reaction direction]] | |||
[[Category: Retro-aldol reaction]] | |||
Revision as of 13:58, 16 February 2022
Designed enzyme RA61 M48K/I72D mutant: form IVDesigned enzyme RA61 M48K/I72D mutant: form IV
Structural highlights
Publication Abstract from PubMedNatural aldolase enzymes and created retro-aldolase protein catalysts often catalyze both aldol and retro-aldol reactions depending on the concentrations of the reactants and the products. Here, we report that the directionality of protein catalysts can be altered by replacing one amino acid. The protein catalyst derived from a scaffold of a previously reported retro-aldolase catalyst, catalyzed aldol reactions more efficiently than the previously reported retro-aldolase catalyst. The retro-aldolase catalyst efficiently catalyzed the retro-aldol reaction but was less efficient in catalyzing the aldol reaction. The results indicate that protein catalysts with varying levels of directionality in usually reversibly catalyzed aldol and retro-aldol reactions can be generated from the same protein scaffold. Varying the Directionality of Protein Catalysts for Aldol and Retro-Aldol Reactions.,Fujioka T, Numoto N, Akama H, Shilpa K, Oka M, Roy PK, Krishna Y, Ito N, Baker D, Oda M, Tanaka F Chembiochem. 2021 Oct 26. doi: 10.1002/cbic.202100435. PMID:34698422[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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