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| [[Image:1foa.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1foa| PDB=1foa | SCENE= }} | | {{STRUCTURE_1foa| PDB=1foa | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINYLTRANSFERASE I'''
| | ===CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINYLTRANSFERASE I=== |
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| ==Overview==
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| N:-acetylglucosaminyltransferase I (GnT I) serves as the gateway from oligomannose to hybrid and complex N:-glycans and plays a critical role in mammalian development and possibly all metazoans. We have determined the X-ray crystal structure of the catalytic fragment of GnT I in the absence and presence of bound UDP-GlcNAc/Mn(2+) at 1.5 and 1.8 A resolution, respectively. The structures identify residues critical for substrate binding and catalysis and provide evidence for similarity, at the mechanistic level, to the deglycosylation step of retaining beta-glycosidases. The structuring of a 13 residue loop, resulting from UDP-GlcNAc/Mn(2+) binding, provides an explanation for the ordered sequential 'Bi Bi' kinetics shown by GnT I. Analysis reveals a domain shared with Bacillus subtilis glycosyltransferase SpsA, bovine beta-1,4-galactosyl transferase 1 and Escherichia coli N:-acetylglucosamine-1-phosphate uridyltransferase. The low sequence identity, conserved fold and related functional features shown by this domain define a superfamily whose members probably share a common ancestor. Sequence analysis and protein threading show that the domain is represented in proteins from several glycosyltransferase families.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11032794}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11032794 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11032794}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Donor substrate and metal ion complex]] | | [[Category: Donor substrate and metal ion complex]] |
| [[Category: N-acetylglucosaminyltransferase i]] | | [[Category: N-acetylglucosaminyltransferase i]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:34:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:40:23 2008'' |