6gp0: Difference between revisions
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<StructureSection load='6gp0' size='340' side='right'caption='[[6gp0]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='6gp0' size='340' side='right'caption='[[6gp0]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6gp0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[6gp0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GP0 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5SQ:2-[2-[(1~{S})-1-AZANYL-2-(1~{H}-IMIDAZOL-4-YL)ETHYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXIDANYLIDENE-IMIDAZOL-1-YL]ETHANOIC+ACID'>5SQ</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene>, <scene name='pdbligand=RC7:{(2R)-4-(4-HYDROXYBENZYL)-2-[2-(1H-IMIDAZOL-4-YL)ETHYL]-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETALDEHYDE'>RC7</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp0 OCA], [https://pdbe.org/6gp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gp0 RCSB], [https://www.ebi.ac.uk/pdbsum/6gp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp0 OCA], [https://pdbe.org/6gp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gp0 RCSB], [https://www.ebi.ac.uk/pdbsum/6gp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Lobophyllia hemprichii]] | ||
[[Category: Adam | [[Category: Adam V]] | ||
[[Category: Bourgeois | [[Category: Bourgeois D]] | ||
[[Category: Byrdin | [[Category: Byrdin M]] | ||
[[Category: | [[Category: De Zitter E]] | ||
[[Category: | [[Category: Dedecker P]] | ||
[[Category: | [[Category: Van Meervelt L]] | ||
Latest revision as of 13:24, 15 November 2023
Structure of mEos4b in the red fluorescent stateStructure of mEos4b in the red fluorescent state
Structural highlights
FunctionPublication Abstract from PubMedGreen-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.,De Zitter E, Thedie D, Monkemoller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D Nat Methods. 2019 Jul 8. pii: 10.1038/s41592-019-0462-3. doi:, 10.1038/s41592-019-0462-3. PMID:31285624[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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