7oxp: Difference between revisions
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==Cryo-EM structure of yeast Sei1== | ==Cryo-EM structure of yeast Sei1== | ||
<StructureSection load='7oxp' size='340' side='right'caption='[[7oxp]]' scene=''> | <StructureSection load='7oxp' size='340' side='right'caption='[[7oxp]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXP FirstGlance]. <br> | <table><tr><td colspan='2'>[[7oxp]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxp OCA], [https://pdbe.org/7oxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxp RCSB], [https://www.ebi.ac.uk/pdbsum/7oxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxp ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxp OCA], [https://pdbe.org/7oxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxp RCSB], [https://www.ebi.ac.uk/pdbsum/7oxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lipid droplets (LDs) are universal lipid storage organelles with a core of neutral lipids, such as triacylglycerols, surrounded by a phospholipid monolayer. This unique architecture is generated during LD biogenesis at endoplasmic reticulum (ER) sites marked by Seipin, a conserved membrane protein mutated in lipodystrophy. Here structural, biochemical and molecular dynamics simulation approaches reveal the mechanism of LD formation by the yeast Seipin Sei1 and its membrane partner Ldb16. We show that Sei1 luminal domain assembles a homooligomeric ring, which, in contrast to other Seipins, is unable to concentrate triacylglycerol. Instead, Sei1 positions Ldb16, which concentrates triacylglycerol within the Sei1 ring through critical hydroxyl residues. Triacylglycerol recruitment to the complex is further promoted by Sei1 transmembrane segments, which also control Ldb16 stability. Thus, we propose that LD assembly by the Sei1/Ldb16 complex, and likely other Seipins, requires sequential triacylglycerol-concentrating steps via distinct elements in the ER membrane and lumen. | |||
Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex.,Klug YA, Deme JC, Corey RA, Renne MF, Stansfeld PJ, Lea SM, Carvalho P Nat Commun. 2021 Oct 8;12(1):5892. doi: 10.1038/s41467-021-26162-6. PMID:34625558<ref>PMID:34625558</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7oxp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Deme | [[Category: Deme, J C]] | ||
[[Category: Lea | [[Category: Lea, S M]] | ||
[[Category: Lipid binding]] | |||
[[Category: Lipid droplet formation]] | |||
[[Category: Membrane protein]] | |||
[[Category: Seipin]] | |||
Latest revision as of 23:17, 20 October 2021
Cryo-EM structure of yeast Sei1Cryo-EM structure of yeast Sei1
Structural highlights
Publication Abstract from PubMedLipid droplets (LDs) are universal lipid storage organelles with a core of neutral lipids, such as triacylglycerols, surrounded by a phospholipid monolayer. This unique architecture is generated during LD biogenesis at endoplasmic reticulum (ER) sites marked by Seipin, a conserved membrane protein mutated in lipodystrophy. Here structural, biochemical and molecular dynamics simulation approaches reveal the mechanism of LD formation by the yeast Seipin Sei1 and its membrane partner Ldb16. We show that Sei1 luminal domain assembles a homooligomeric ring, which, in contrast to other Seipins, is unable to concentrate triacylglycerol. Instead, Sei1 positions Ldb16, which concentrates triacylglycerol within the Sei1 ring through critical hydroxyl residues. Triacylglycerol recruitment to the complex is further promoted by Sei1 transmembrane segments, which also control Ldb16 stability. Thus, we propose that LD assembly by the Sei1/Ldb16 complex, and likely other Seipins, requires sequential triacylglycerol-concentrating steps via distinct elements in the ER membrane and lumen. Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex.,Klug YA, Deme JC, Corey RA, Renne MF, Stansfeld PJ, Lea SM, Carvalho P Nat Commun. 2021 Oct 8;12(1):5892. doi: 10.1038/s41467-021-26162-6. PMID:34625558[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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