2a6j: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2a6j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6J FirstGlance]. <br> | <table><tr><td colspan='2'>[[2a6j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6J FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6j OCA], [https://pdbe.org/2a6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6j RCSB], [https://www.ebi.ac.uk/pdbsum/2a6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6j ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6j OCA], [https://pdbe.org/2a6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6j RCSB], [https://www.ebi.ac.uk/pdbsum/2a6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Agarwal | [[Category: Agarwal A]] | ||
[[Category: Manivel | [[Category: Manivel V]] | ||
[[Category: Rao | [[Category: Rao KV]] | ||
[[Category: Salunke | [[Category: Salunke DM]] | ||
[[Category: Sethi | [[Category: Sethi DK]] | ||
Revision as of 11:15, 15 May 2024
Crystal structure analysis of the anti-arsonate germline antibody 36-65Crystal structure analysis of the anti-arsonate germline antibody 36-65
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCorrelation between the promiscuity of the primary antibody response and conformational flexibility in a germline antibody was addressed by using germline antibody 36-65. Crystallographic analyses of the 36-65 Fab with three independent dodecapeptides provided mechanistic insights into the generation of antibody diversity. While four antigen-free Fab molecules provided a quantitative description of the conformational repertoire of the antibody CDRs, three Fab molecules bound to structurally diverse peptide epitopes exhibited a common paratope conformation. Each peptide revealed spatially different footprints within the antigen-combining site. However, a conformation-specific lock involving two shared residues, which were also associated with hapten binding, was discernible. Unlike the hapten, the peptides interacted with residues that undergo somatic mutations, suggesting a possible mechanism for excluding "nonspecific" antigens during affinity maturation. The observed multiple binding modes of diverse epitopes within a common paratope conformation of a germline antibody reveal a simple, yet elegant, mechanism for expanding the primary antibody repertoire. Differential epitope positioning within the germline antibody paratope enhances promiscuity in the primary immune response.,Sethi DK, Agarwal A, Manivel V, Rao KV, Salunke DM Immunity. 2006 Apr;24(4):429-38. PMID:16618601[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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