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==NMR AND RESTRAINED MOLECULAR DYNAMICS STUDY OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF TOXIN FS2, A SPECIFIC BLOCKER OF THE L-TYPE CALCIUM CHANNEL, ISOLATED FROM BLACK MAMBA VENOM==
==NMR AND RESTRAINED MOLECULAR DYNAMICS STUDY OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF TOXIN FS2, A SPECIFIC BLOCKER OF THE L-TYPE CALCIUM CHANNEL, ISOLATED FROM BLACK MAMBA VENOM==
<StructureSection load='1tfs' size='340' side='right'caption='[[1tfs]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1tfs' size='340' side='right'caption='[[1tfs]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1tfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Black_mamba Black mamba]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TFS FirstGlance]. <br>
<table><tr><td colspan='2'>[[1tfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendroaspis_polylepis_polylepis Dendroaspis polylepis polylepis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TFS FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tfs OCA], [https://pdbe.org/1tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tfs RCSB], [https://www.ebi.ac.uk/pdbsum/1tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tfs ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tfs OCA], [https://pdbe.org/1tfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tfs RCSB], [https://www.ebi.ac.uk/pdbsum/1tfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tfs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/TXFS2_DENPO TXFS2_DENPO]] Specific blocker of the voltage-dependent L-type calcium channel (Cav1/CACNA1).<ref>PMID:8826098</ref> <ref>PMID:9636051</ref> 
[https://www.uniprot.org/uniprot/3SL2_DENPO 3SL2_DENPO] Specific blocker of the voltage-dependent L-type calcium channel (Cav1/CACNA1) (PubMed:8826098). Inhibits cardiac contractions (By similarity).[UniProtKB:P22947]<ref>PMID:8826098</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tfs ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tfs ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional solution structure of toxin FS2, a 60-residue polypeptide isolated from the venom of black mamba snake (Dendroaspis polylepis polylepis), has been determined by nuclear magnetic resonance spectroscopy. Using 600 NOE constraints and 55 dihedral angle constraints, a set of 20 structures obtained from distance-geometry calculations was further refined by molecular dynamics calculations using a combined simulated annealing-restrained MD protocol. The resulting 20 conformers, taken to represent the solution structure, give an average rmsd of 1.2 A for their backbone atoms, relative to the average structure. The overall resulting three-fingered structure is similar to those already observed in several postsynaptic neurotoxins, cardiotoxins, and fasciculins, which all share with toxin FS2 the same network of four disulfide bridges. The overall concavity of the molecule, considered as a flat bottomed dish, is oriented toward the C-terminal loop of the molecule. This orientation is similar to that of fasciculins and cardiotoxins but opposite to that of neurotoxins. On the basis of the local rms displacements between the 20 conformers, the structure of the first loop appears to be less well defined in FS2 than in the previously reported neurotoxin structures, but fasciculin 1 shows a similar trend with particularly high temperature factors for this part of the X-ray structure. The concave side which presents most of the positively charged residues is quite similar in FS2 and fasciculin 1. The main difference is shown by the convex side of the third loop, mostly hydrophobic in FS2, in contrast to the pair of negatively charged aspartates in fasciculin 1. This difference could be one of the factors leading to the distinct pharmacological properties-L-type calcium channel blocker for FS2 and cholinesterase inhibitor for fasciculin--observed for these two subgroups of the "angusticeps-type" toxins.
NMR and restrained molecular dynamics study of the three-dimensional solution structure of toxin FS2, a specific blocker of the L-type calcium channel, isolated from black mamba venom.,Albrand JP, Blackledge MJ, Pascaud F, Hollecker M, Marion D Biochemistry. 1995 May 2;34(17):5923-37. PMID:7727450<ref>PMID:7727450</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1tfs" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Black mamba]]
[[Category: Dendroaspis polylepis polylepis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Albrand, J P]]
[[Category: Albrand J-P]]
[[Category: Blackledge, M J]]
[[Category: Blackledge MJ]]
[[Category: Hollecker, M]]
[[Category: Hollecker M]]
[[Category: Marion, D]]
[[Category: Marion D]]
[[Category: Pascaud, F]]
[[Category: Pascaud F]]
[[Category: Toxin]]

Revision as of 11:40, 1 May 2024

NMR AND RESTRAINED MOLECULAR DYNAMICS STUDY OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF TOXIN FS2, A SPECIFIC BLOCKER OF THE L-TYPE CALCIUM CHANNEL, ISOLATED FROM BLACK MAMBA VENOMNMR AND RESTRAINED MOLECULAR DYNAMICS STUDY OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF TOXIN FS2, A SPECIFIC BLOCKER OF THE L-TYPE CALCIUM CHANNEL, ISOLATED FROM BLACK MAMBA VENOM

Structural highlights

1tfs is a 1 chain structure with sequence from Dendroaspis polylepis polylepis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

3SL2_DENPO Specific blocker of the voltage-dependent L-type calcium channel (Cav1/CACNA1) (PubMed:8826098). Inhibits cardiac contractions (By similarity).[UniProtKB:P22947][1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Yasuda O, Morimoto S, Jiang B, Kuroda H, Kimura T, Sakakibara S, Fukuo K, Chen S, Tamatani M, Ogihara T. FS2. a mamba venom toxin, is a specific blocker of the L-type calcium channels. Artery. 1994;21(5):287-302. PMID:8826098
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