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==Cryo-EM Structure of the Sodium-driven Chloride/Bicarbonate Exchanger NDCBE (SLC4A8)== | ==Cryo-EM Structure of the Sodium-driven Chloride/Bicarbonate Exchanger NDCBE (SLC4A8)== | ||
<StructureSection load='7rtm' size='340' side='right'caption='[[7rtm]]' scene=''> | <StructureSection load='7rtm' size='340' side='right'caption='[[7rtm]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTM FirstGlance]. <br> | <table><tr><td colspan='2'>[[7rtm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTM FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtm OCA], [https://pdbe.org/7rtm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtm RCSB], [https://www.ebi.ac.uk/pdbsum/7rtm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtm ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtm OCA], [https://pdbe.org/7rtm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtm RCSB], [https://www.ebi.ac.uk/pdbsum/7rtm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtm ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
SLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) transporters revealed an identical overall fold despite their different transport modes (chloride/bicarbonate exchange versus sodium-carbonate cotransport). However, the exact mechanism determining the different transport modes in the SLC4 family remains unknown. In this work, we report the cryo-EM 3.4 A structure of the OF conformation of NDCBE (SLC4A8), which shares transport properties with both AE1 and NBCe1 by mediating the electroneutral exchange of sodium-carbonate with chloride. This structure features a fully resolved extracellular loop 3 and well-defined densities corresponding to sodium and carbonate ions in the tentative substrate binding pocket. Further, we combine computational modeling with functional studies to unravel the molecular determinants involved in NDCBE and SLC4 transport. | |||
Cryo-EM structure of the sodium-driven chloride/bicarbonate exchanger NDCBE.,Wang W, Tsirulnikov K, Zhekova HR, Kayik G, Khan HM, Azimov R, Abuladze N, Kao L, Newman D, Noskov SY, Zhou ZH, Pushkin A, Kurtz I Nat Commun. 2021 Sep 28;12(1):5690. doi: 10.1038/s41467-021-25998-2. PMID:34584093<ref>PMID:34584093</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7rtm" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Abuladze N]] | [[Category: Abuladze, N]] | ||
[[Category: Azimov R]] | [[Category: Azimov, R]] | ||
[[Category: Kao L]] | [[Category: Kao, L]] | ||
[[Category: Kayik G]] | [[Category: Kayik, G]] | ||
[[Category: Kurtz I]] | [[Category: Kurtz, I]] | ||
[[Category: Muhammad-Khan H]] | [[Category: Muhammad-Khan, H]] | ||
[[Category: Newman D]] | [[Category: Newman, D]] | ||
[[Category: Noskov | [[Category: Noskov, S Y]] | ||
[[Category: Pushkin A]] | [[Category: Pushkin, A]] | ||
[[Category: Tsirulnikov K]] | [[Category: Tsirulnikov, K]] | ||
[[Category: Wang | [[Category: Wang, W G]] | ||
[[Category: Zhekova H]] | [[Category: Zhekova, H]] | ||
[[Category: Zhou | [[Category: Zhou, Z H]] | ||
[[Category: Cryo-em]] | |||
[[Category: Membrane protein]] | |||
[[Category: Ndcbe]] | |||
[[Category: Sodium-driven chloride/bicarbonate exchanger]] | |||
Revision as of 08:57, 6 October 2021
Cryo-EM Structure of the Sodium-driven Chloride/Bicarbonate Exchanger NDCBE (SLC4A8)Cryo-EM Structure of the Sodium-driven Chloride/Bicarbonate Exchanger NDCBE (SLC4A8)
Structural highlights
Publication Abstract from PubMedSLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) transporters revealed an identical overall fold despite their different transport modes (chloride/bicarbonate exchange versus sodium-carbonate cotransport). However, the exact mechanism determining the different transport modes in the SLC4 family remains unknown. In this work, we report the cryo-EM 3.4 A structure of the OF conformation of NDCBE (SLC4A8), which shares transport properties with both AE1 and NBCe1 by mediating the electroneutral exchange of sodium-carbonate with chloride. This structure features a fully resolved extracellular loop 3 and well-defined densities corresponding to sodium and carbonate ions in the tentative substrate binding pocket. Further, we combine computational modeling with functional studies to unravel the molecular determinants involved in NDCBE and SLC4 transport. Cryo-EM structure of the sodium-driven chloride/bicarbonate exchanger NDCBE.,Wang W, Tsirulnikov K, Zhekova HR, Kayik G, Khan HM, Azimov R, Abuladze N, Kao L, Newman D, Noskov SY, Zhou ZH, Pushkin A, Kurtz I Nat Commun. 2021 Sep 28;12(1):5690. doi: 10.1038/s41467-021-25998-2. PMID:34584093[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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