1sjv: Difference between revisions

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<StructureSection load='1sjv' size='340' side='right'caption='[[1sjv]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
<StructureSection load='1sjv' size='340' side='right'caption='[[1sjv]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1sjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJV FirstGlance]. <br>
<table><tr><td colspan='2'>[[1sjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjv OCA], [https://pdbe.org/1sjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjv RCSB], [https://www.ebi.ac.uk/pdbsum/1sjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjv ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjv OCA], [https://pdbe.org/1sjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjv RCSB], [https://www.ebi.ac.uk/pdbsum/1sjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjv ProSAT]</span></td></tr>
</table>
</table>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjv ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjv ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Among mammals, camelids have a unique immunological system since they produce functional antibodies devoid of light chains and CH1 domains. To bind antigens, whether they are proteins or haptens, camelids use the single domain VH from their heavy chain (VHH). We report here on such a llama VHH domain (VHH-R9) which was raised against a hapten, the RR6 red dye. This VHH possesses the shortest complementarity determining region 3 (CDR3) among all the known VHH sequences and nevertheless binds RR6 efficiently with a K(d) value of 83 nM. However, the crystal structure of VHH-R9 exhibits a striking feature: its CDR3 and its last beta-strand (beta9) do not follow the immunoglobulin VH domain fold, but instead extend out of the VHH molecular boundary and associate with a symmetry-related molecule. The two monomers thus form a domain-swapped dimer which establishes further contacts with symmetry-related molecules and build a crystal-wide beta-sheet structure. The driving force of the dimer formation is probably the strain induced by the short CDR3 together with the cleavage of the first seven residues.
Domain swapping of a llama VHH domain builds a crystal-wide beta-sheet structure.,Spinelli S, Desmyter A, Frenken L, Verrips T, Tegoni M, Cambillau C FEBS Lett. 2004 Apr 23;564(1-2):35-40. PMID:15094039<ref>PMID:15094039</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1sjv" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Camelus glama]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cambillau, C]]
[[Category: Cambillau C]]
[[Category: Desmyter, A]]
[[Category: Desmyter A]]
[[Category: Frenken, L]]
[[Category: Frenken L]]
[[Category: Spinelli, S]]
[[Category: Spinelli S]]
[[Category: Verrips, T]]
[[Category: Verrips T]]
[[Category: Antibiotic]]
[[Category: Camelids antibody]]
[[Category: Domain swapping]]
[[Category: Heavy chain antibody]]

Revision as of 11:32, 1 May 2024

Three-Dimensional Structure of a Llama VHH Domain SwappingThree-Dimensional Structure of a Llama VHH Domain Swapping

Structural highlights

1sjv is a 1 chain structure with sequence from Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.94Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1sjv, resolution 1.94Å

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