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==== | ==Crystal structure of the variable region of Plasmodium RIFIN #4 (PF3D7_1000500) in complex with LAIR1 (with T67L, N69S and A77T mutations)== | ||
<StructureSection load='7f9m' size='340' side='right'caption='[[7f9m]]' scene=''> | <StructureSection load='7f9m' size='340' side='right'caption='[[7f9m]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7f9m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F9M FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f9m OCA], [https://pdbe.org/7f9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f9m RCSB], [https://www.ebi.ac.uk/pdbsum/7f9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f9m ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f9m OCA], [https://pdbe.org/7f9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f9m RCSB], [https://www.ebi.ac.uk/pdbsum/7f9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f9m ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A143ZWD5_PLAF7 A0A143ZWD5_PLAF7] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Malaria infection by Plasmodium falciparum continues to pose a global threat to the human population. P. falciparum expresses variable erythrocyte surface antigens such as RIFINs. Public antibodies with LAIR1 insertion have been identified from malarial patients against a subset of RIFINs. In this study, we solve a LAIR1-binding RIFIN structure: the complex structures of two RIFINs bound to mutated or wild-type LAIR1 in two distinct patterns. Notably, the two RIFINs engage similar binding sites on LAIR1 with different angles, and the RIFIN-binding sites overlap with the collagen-binding site. Surprisingly, RIFINs use completely different binding sites to bind to LAIR1 or LILRB1, indicating the kaleidoscopic change of RIFINs. We then verify that RIFIN could induce LAIR1-mediated cell signaling, and LAIR1-containing antibodies could block the pathway. The findings of this study provide structural insights into the mechanism of the immune escape of P. falciparum and the endless arms race between parasite and host. | |||
Structural basis of malarial parasite RIFIN-mediated immune escape against LAIR1.,Xie Y, Li X, Chai Y, Song H, Qi J, Gao GF Cell Rep. 2021 Aug 24;36(8):109600. doi: 10.1016/j.celrep.2021.109600. PMID:34433057<ref>PMID:34433057</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7f9m" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Leukocyte-associated immunoglobulin-like receptor|Leukocyte-associated immunoglobulin-like receptor]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Plasmodium falciparum 3D7]] | ||
[[Category: Gao GF]] | |||
[[Category: Li X]] | |||
[[Category: Qi J]] | |||
[[Category: Song H]] | |||
[[Category: Xie Y]] | |||
Revision as of 20:11, 29 November 2023
Crystal structure of the variable region of Plasmodium RIFIN #4 (PF3D7_1000500) in complex with LAIR1 (with T67L, N69S and A77T mutations)Crystal structure of the variable region of Plasmodium RIFIN #4 (PF3D7_1000500) in complex with LAIR1 (with T67L, N69S and A77T mutations)
Structural highlights
FunctionPublication Abstract from PubMedMalaria infection by Plasmodium falciparum continues to pose a global threat to the human population. P. falciparum expresses variable erythrocyte surface antigens such as RIFINs. Public antibodies with LAIR1 insertion have been identified from malarial patients against a subset of RIFINs. In this study, we solve a LAIR1-binding RIFIN structure: the complex structures of two RIFINs bound to mutated or wild-type LAIR1 in two distinct patterns. Notably, the two RIFINs engage similar binding sites on LAIR1 with different angles, and the RIFIN-binding sites overlap with the collagen-binding site. Surprisingly, RIFINs use completely different binding sites to bind to LAIR1 or LILRB1, indicating the kaleidoscopic change of RIFINs. We then verify that RIFIN could induce LAIR1-mediated cell signaling, and LAIR1-containing antibodies could block the pathway. The findings of this study provide structural insights into the mechanism of the immune escape of P. falciparum and the endless arms race between parasite and host. Structural basis of malarial parasite RIFIN-mediated immune escape against LAIR1.,Xie Y, Li X, Chai Y, Song H, Qi J, Gao GF Cell Rep. 2021 Aug 24;36(8):109600. doi: 10.1016/j.celrep.2021.109600. PMID:34433057[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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