7ecg: Difference between revisions
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==Crystal Structure of d(G4C2)2-Ba in F222 space group== | ==Crystal Structure of d(G4C2)2-Ba in F222 space group== | ||
<StructureSection load='7ecg' size='340' side='right'caption='[[7ecg]]' scene=''> | <StructureSection load='7ecg' size='340' side='right'caption='[[7ecg]], [[Resolution|resolution]] 1.97Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ECG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ECG FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ecg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ECG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ECG FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ecg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ecg OCA], [https://pdbe.org/7ecg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ecg RCSB], [https://www.ebi.ac.uk/pdbsum/7ecg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ecg ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ecg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ecg OCA], [https://pdbe.org/7ecg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ecg RCSB], [https://www.ebi.ac.uk/pdbsum/7ecg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ecg ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The hexanucleotide repeat expansion, GGGGCC (G4C2), within the first intron of the C9orf72 gene is known to be the most common genetic cause of both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The G4C2 repeat expansions, either DNA or RNA, are able to form G-quadruplexes which induce toxicity leading to ALS/FTD. Herein, we report a novel crystal structure of d(G4C2)2 that self-associates to form an eight-layer parallel tetrameric G-quadruplex. Two d(G4C2)2 associate together as a parallel dimeric G-quadruplex which folds into a tetramer via 5'-to-5' arrangements. Each dimer consists of four G-tetrads connected by two CC propeller loops. Especially, the 3'-end cytosines protrude out and form C.C+*C.C+/ C.C*C.C+ quadruple base pair or C*C.C+ triple base pair stacking on the dimeric block. Our work sheds light on the G-quadruplexes adopted by d(G4C2) and yields the invaluable structural details for the development of small molecules to tackle neurodegenerative diseases, ALS and FTD. | |||
Crystal structure of parallel G-quadruplex formed by the two-repeat ALS- and FTD-related GGGGCC sequence.,Geng Y, Liu C, Cai Q, Luo Z, Miao H, Shi X, Xu N, Fung CP, Choy TT, Yan B, Li N, Qian P, Zhou B, Zhu G Nucleic Acids Res. 2021 May 28. pii: 6287844. doi: 10.1093/nar/gkab302. PMID:34048588<ref>PMID:34048588</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ecg" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cai Q]] | [[Category: Cai, Q]] | ||
[[Category: Geng Y]] | [[Category: Geng, Y]] | ||
[[Category: Liu C]] | [[Category: Liu, C]] | ||
[[Category: Luo Z]] | [[Category: Luo, Z]] | ||
[[Category: Zhu G]] | [[Category: Zhu, G]] | ||
[[Category: Dna]] | |||
[[Category: G-quadruplex]] | |||
Revision as of 10:48, 25 June 2021
Crystal Structure of d(G4C2)2-Ba in F222 space groupCrystal Structure of d(G4C2)2-Ba in F222 space group
Structural highlights
Publication Abstract from PubMedThe hexanucleotide repeat expansion, GGGGCC (G4C2), within the first intron of the C9orf72 gene is known to be the most common genetic cause of both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The G4C2 repeat expansions, either DNA or RNA, are able to form G-quadruplexes which induce toxicity leading to ALS/FTD. Herein, we report a novel crystal structure of d(G4C2)2 that self-associates to form an eight-layer parallel tetrameric G-quadruplex. Two d(G4C2)2 associate together as a parallel dimeric G-quadruplex which folds into a tetramer via 5'-to-5' arrangements. Each dimer consists of four G-tetrads connected by two CC propeller loops. Especially, the 3'-end cytosines protrude out and form C.C+*C.C+/ C.C*C.C+ quadruple base pair or C*C.C+ triple base pair stacking on the dimeric block. Our work sheds light on the G-quadruplexes adopted by d(G4C2) and yields the invaluable structural details for the development of small molecules to tackle neurodegenerative diseases, ALS and FTD. Crystal structure of parallel G-quadruplex formed by the two-repeat ALS- and FTD-related GGGGCC sequence.,Geng Y, Liu C, Cai Q, Luo Z, Miao H, Shi X, Xu N, Fung CP, Choy TT, Yan B, Li N, Qian P, Zhou B, Zhu G Nucleic Acids Res. 2021 May 28. pii: 6287844. doi: 10.1093/nar/gkab302. PMID:34048588[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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