1ayv: Difference between revisions
New page: left|200px<br /> <applet load="1ayv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ayv, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF... |
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caption="1ayv, resolution 2.3Å" /> | caption="1ayv, resolution 2.3Å" /> | ||
'''CRYSTAL STRUCTURE OF CYSTEINE PROTEASE HUMAN CATHEPSIN K IN COMPLEX WITH A COVALENT THIAZOLHYDRAZIDE INHIBITOR'''<br /> | '''CRYSTAL STRUCTURE OF CYSTEINE PROTEASE HUMAN CATHEPSIN K IN COMPLEX WITH A COVALENT THIAZOLHYDRAZIDE INHIBITOR'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1AYV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with IN6 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cathepsin_K Cathepsin K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.38 3.4.22.38] Full crystallographic information is available from [http:// | 1AYV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=IN6:'>IN6</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cathepsin_K Cathepsin K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.38 3.4.22.38] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: sulfhydryl proteinase]] | [[Category: sulfhydryl proteinase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:30:15 2008'' | ||
Revision as of 16:30, 15 February 2008
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CRYSTAL STRUCTURE OF CYSTEINE PROTEASE HUMAN CATHEPSIN K IN COMPLEX WITH A COVALENT THIAZOLHYDRAZIDE INHIBITOR
OverviewOverview
Potent and selective active-site-spanning inhibitors have been designed, for cathepsin K, a cysteine protease unique to osteoclasts. They act by, mechanisms that involve tight binding intermediates, potentially on a, hydrolytic pathway. X-ray crystallographic, MS, NMR spectroscopic, and, kinetic studies of the mechanisms of inhibition indicate that different, intermediates or transition states are being represented that are, dependent on the conditions of measurement and the specific groups, flanking the carbonyl in the inhibitor. The species observed, crystallographically are most consistent with tetrahedral intermediates, that may be close approximations of those that occur during substrate, hydrolysis. Initial kinetic studies suggest the possibility of, irreversible and reversible active-site modification. Representative, inhibitors have demonstrated antiresorptive activity both in vitro and in, vivo and therefore are promising leads for therapeutic agents for the, treatment of osteoporosis. Expansion of these inhibitor concepts can be, envisioned for the many other cysteine proteases implicated for, therapeutic intervention.
DiseaseDisease
Known disease associated with this structure: Pycnodysostosis OMIM:[601105]
About this StructureAbout this Structure
1AYV is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Cathepsin K, with EC number 3.4.22.38 Full crystallographic information is available from OCA.
ReferenceReference
Design of potent and selective human cathepsin K inhibitors that span the active site., Thompson SK, Halbert SM, Bossard MJ, Tomaszek TA, Levy MA, Zhao B, Smith WW, Abdel-Meguid SS, Janson CA, D'Alessio KJ, McQueney MS, Amegadzie BY, Hanning CR, DesJarlais RL, Briand J, Sarkar SK, Huddleston MJ, Ijames CF, Carr SA, Garnes KT, Shu A, Heys JR, Bradbeer J, Zembryki D, Lee-Rykaczewski L, James IE, Lark MW, Drake FH, Gowen M, Gleason JG, Veber DF, Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14249-54. PMID:9405598
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