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==Solution structure of Ph1500: a homohexameric protein centered on a 12-bladed beta-propeller==
==Solution structure of Ph1500: a homohexameric protein centered on a 12-bladed beta-propeller==
<StructureSection load='2m3x' size='340' side='right'caption='[[2m3x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2m3x' size='340' side='right'caption='[[2m3x]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2m3x]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M3X FirstGlance]. <br>
<table><tr><td colspan='2'>[[2m3x]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M3X FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jv2|2jv2]]</div></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3x OCA], [https://pdbe.org/2m3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m3x RCSB], [https://www.ebi.ac.uk/pdbsum/2m3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3x ProSAT]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PH1500 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m3x OCA], [https://pdbe.org/2m3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m3x RCSB], [https://www.ebi.ac.uk/pdbsum/2m3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m3x ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/O59169_PYRHO O59169_PYRHO]
Ph1500 is a homohexameric, two-domain protein of unknown function from the hyperthermophilic archaeon Pyrococcus horikoshii. The C-terminal hexamerization domain (Ph1500C) is of particular interest, as it lacks sequence homology to proteins of known structure. However, it resisted crystallization for X-ray analysis, and proteins of this size (49 kDa) present a considerable challenge to NMR structure determination in solution. We solved the high-resolution structure of Ph1500C, exploiting the hyperthermophilic nature of the protein to minimize unfavorable relaxation properties by high-temperature measurement. Thus, the side chain assignment (97%) and structure determination became possible at full proton density. To our knowledge, Ph1500C is the largest protein for which this has been achieved. To minimize detrimental fast water exchange of amide protons at increased temperature, we employed a strategy where the temperature was optimized separately for backbone and side chain experiments.
 
Optimized measurement temperature gives access to the solution structure of a 49 kDa homohexameric beta-propeller.,Varnay I, Truffault V, Djuranovic S, Ursinus A, Coles M, Kessler H J Am Chem Soc. 2010 Nov 10;132(44):15692-8. doi: 10.1021/ja1064608. PMID:20961124<ref>PMID:20961124</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2m3x" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii OT3]]
[[Category: Coles, M]]
[[Category: Coles M]]
[[Category: Kessler, H]]
[[Category: Kessler H]]
[[Category: Truffault, V]]
[[Category: Truffault V]]
[[Category: Varnay, I]]
[[Category: Varnay I]]
[[Category: 12-bladed]]
[[Category: Beta-clam]]
[[Category: Beta-propeller]]
[[Category: Homohexamer]]
[[Category: Unknown function]]

Revision as of 16:28, 22 February 2023

Solution structure of Ph1500: a homohexameric protein centered on a 12-bladed beta-propellerSolution structure of Ph1500: a homohexameric protein centered on a 12-bladed beta-propeller

Structural highlights

2m3x is a 6 chain structure with sequence from Pyrococcus horikoshii OT3. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O59169_PYRHO

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