1eam: Difference between revisions

Revision as of 00:24, 1 July 2008

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File:1eam.png

Template:STRUCTURE 1eam

VACCINIA METHYLTRANSFERASE VP39 MUTANT (EC: 2.7.7.19)VACCINIA METHYLTRANSFERASE VP39 MUTANT (EC: 2.7.7.19)

Template:ABSTRACT PUBMED 10377383

About this StructureAbout this Structure

1EAM is a Single protein structure of sequence from Vaccinia virus. Full crystallographic information is available from OCA.

ReferenceReference

mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains., Hu G, Gershon PD, Hodel AE, Quiocho FA, Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7149-54. PMID:10377383

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''VACCINIA METHYLTRANSFERASE VP39 MUTANT (EC: 2.7.7.19)'''
+===VACCINIA METHYLTRANSFERASE VP39 MUTANT (EC: 2.7.7.19)===
-==Overview==
+<!--
-We have determined, by high resolution x-ray analysis, 10 structures comprising the mRNA cap-specific methyltransferase VP39 or specific mutants thereof in the presence of methylated nucleobase analogs (N1-methyladenine, N3-methyladenine, N1-methylcytosine, N3-methylcytosine) and their unmethylated counterparts, or nucleoside N7-methylguanosine. Together with solution affinity studies and previous crystallographic data for N7-methylguanosine and its phosphorylated derivatives, these data demonstrate that only methylated, positively charged bases are bound, indicating that their enhanced stacking with two aromatic side chains of VP39 (Tyr 22 and Phe 180) plays a dominant role in cap recognition. Four key features characterize this stacking interaction: (i) near perfect parallel alignment between the sandwiched methylated bases and aromatic side chains, (ii) substantial areas of overlap in the two-stacked rings, (iii) a 3.4-A interplanar spacing within the overlapping region, and (iv) positive charge in the heterocyclic nucleobase.
+The line below this paragraph, {{ABSTRACT_PUBMED_10377383}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10377383 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10377383}}
 ==About this Structure==
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 [[Category: Transcription]]
 [[Category: Vaccinia]]
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