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| {{STRUCTURE_1e7p| PDB=1e7p | SCENE= }} | | {{STRUCTURE_1e7p| PDB=1e7p | SCENE= }} |
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| '''QUINOL:FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES'''
| | ===QUINOL:FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES=== |
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| ==Overview==
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| Quinol:fumarate reductase (QFR) is a membrane protein complex that couples the reduction of fumarate to succinate to the oxidation of quinol to quinone. Previously, the crystal structure of QFR from Wolinella succinogenes was determined based on two different crystal forms, and the site of fumarate binding in the flavoprotein subunit A of the enzyme was located between the FAD-binding domain and the capping domain [Lancaster, C.R.D., Kroger, A., Auer, M., & Michel, H. (1999) Nature 402, 377--385]. Here we describe the structure of W. succinogenes QFR based on a third crystal form and refined at 3.1 A resolution. Compared with the previous crystal forms, the capping domain is rotated in this structure by approximately 14 degrees relative to the FAD-binding domain. As a consequence, the topology of the dicarboxylate binding site is much more similar to those of membrane-bound and soluble fumarate reductase enzymes from other organisms than to that found in the previous crystal forms of W. succinogenes QFR. This and the effects of the replacement of Arg A301 by Glu or Lys by site-directed mutagenesis strongly support a common mechanism for fumarate reduction in this superfamily of enzymes.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11248702}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11248702 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11248702}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Respiratory chain]] | | [[Category: Respiratory chain]] |
| [[Category: Succinate dehydrogenase]] | | [[Category: Succinate dehydrogenase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:46:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:17:23 2008'' |