1e4p: Difference between revisions
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==Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site== | ==Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site== | ||
<StructureSection load='1e4p' size='340' side='right'caption='[[1e4p | <StructureSection load='1e4p' size='340' side='right'caption='[[1e4p]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1e4p]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E4P FirstGlance]. <br> | <table><tr><td colspan='2'>[[1e4p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora Neurospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E4P FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4p OCA], [https://pdbe.org/1e4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e4p RCSB], [https://www.ebi.ac.uk/pdbsum/1e4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e4p ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4p OCA], [https://pdbe.org/1e4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e4p RCSB], [https://www.ebi.ac.uk/pdbsum/1e4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e4p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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==See Also== | ==See Also== | ||
*[[Ribozyme|Ribozyme]] | *[[Ribozyme 3D structures|Ribozyme 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Neurospora]] | ||
[[Category: | [[Category: Heus HA]] | ||
[[Category: | [[Category: Hilbers CW]] | ||
[[Category: | [[Category: Michiels PJA]] | ||
[[Category: | [[Category: Schouten CHJ]] | ||
Revision as of 13:22, 21 December 2022
Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage siteStructure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site
Structural highlights
Publication Abstract from PubMedThe cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure. Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site.,Michiels PJ, Schouten CH, Hilbers CW, Heus HA RNA. 2000 Dec;6(12):1821-32. PMID:11142381[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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