7d7m: Difference between revisions

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-====
+==Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein==
-<StructureSection load='7d7m' size='340' side='right'caption='[[7d7m]]' scene=''>
+<StructureSection load='7d7m' size='340' side='right'caption='[[7d7m]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7d7m]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D7M FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d7m OCA], [https://pdbe.org/7d7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d7m RCSB], [https://www.ebi.ac.uk/pdbsum/7d7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d7m ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P2E:(Z)-7-[(1R,2R,3R)-3-HYDROXY-2-[(E,3S)-3-HYDROXYOCT-1-ENYL]-5-OXO-CYCLOPENTYL]HEPT-5-ENOIC+ACID'>P2E</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d7m OCA], [https://pdbe.org/7d7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d7m RCSB], [https://www.ebi.ac.uk/pdbsum/7d7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d7m ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PE2R4_HUMAN PE2R4_HUMAN] Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Prostaglandin E receptor EP4, a class A G protein-coupled receptor (GPCR), is a common drug target in various disorders, such as acute decompensated heart failure and ulcerative colitis. Here, we report the cryoelectron microscopy (cryo-EM) structure of the EP4-heterotrimeric G protein (Gs) complex with the endogenous ligand at a global resolution of 3.3 A. In this structure, compared with that in the inactive EP4 structure, the sixth transmembrane domain is shifted outward on the intracellular side, although the shift is smaller than that in other class A GPCRs bound to Gs. Instead, the C-terminal helix of Gs is inserted toward TM2 of EP4, and the conserved C-terminal hook structure formsthe extended state. These structural features are formed by the conserved residues in prostanoid receptors (Phe54(2.39) and Trp327(7.51)). These findings may be important for the thorough understanding of the G protein-binding mechanism of EP4 and other prostanoid receptors.
+Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein.,Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H, Kobayashi T Structure. 2021 Mar 4;29(3):252-260.e6. doi: 10.1016/j.str.2020.11.007. Epub 2020 , Dec 1. PMID:33264604<ref>PMID:33264604</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 7d7m" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Transducin 3D structures|Transducin 3D structures]]
+== References ==
+<references/>
 __TOC__
 </StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Lama glama]]
 [[Category: Large Structures]]
-[[Category: Z-disk]]
+[[Category: Fujita Y]]
+[[Category: Iwata S]]
+[[Category: Kimura TK]]
+[[Category: Kobayashi T]]
+[[Category: Morimoto K]]
+[[Category: Noda T]]
+[[Category: Nojima S]]
+[[Category: Nomura N]]
+[[Category: Shigematsu H]]
+[[Category: Suno R]]
+[[Category: Yamamoto M]]