'''STUDY ON RADIATION DAMAGE ON A CRYOCOOLED CRYSTAL. REFINED PART 6: STRUCTURE AFTER A RADIATION DOSE OF 54*10E15 PHOTON'''
===STUDY ON RADIATION DAMAGE ON A CRYOCOOLED CRYSTAL. REFINED PART 6: STRUCTURE AFTER A RADIATION DOSE OF 54*10E15 PHOTON===
==Overview==
<!--
The high intensity of third-generation X-ray sources, along with the development of cryo-cooling of protein crystals at temperatures around 100 K, have made it possible to extend the diffraction limit of crystals and to reduce their size. However, even with cryo-cooled crystals, radiation damage becomes a limiting factor. So far, the radiation damage has manifested itself in the form of a loss of overall diffracted intensity and an increase in the temperature factor. The structure of a protein (myrosinase) after exposure to different doses of X-rays in the region of 20 x 10(15) photons mm(-2) has been studied. The changes in the structure owing to radiation damage were analysed using Fourier difference maps and occupancy refinement for the first time. Damage was obvious in the form of breakage of disulfide bonds, decarboxylation of aspartate and glutamate residues, a loss of hydroxyl groups from tyrosine and of the methylthio group of methionine. The susceptibility to radiation damage of individual groups of the same kind varies within the protein. The quality of the model resulting from structure determination might be compromised owing to the presence of radiolysis in the crystal after an excessive radiation dose. Radiation-induced structural changes may interfere with the interpretation of ligand-binding studies or MAD data. The experiments reported here suggest that there is an intrinsic limit to the amount of data which can be extracted from a sample of a given size.
The line below this paragraph, {{ABSTRACT_PUBMED_10713520}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10713520 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_10713520}}
==About this Structure==
==About this Structure==
Line 27:
Line 31:
[[Category: Radiation damage]]
[[Category: Radiation damage]]
[[Category: Radiolysis]]
[[Category: Radiolysis]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:21:53 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:43:27 2008''
Revision as of 23:43, 30 June 2008
This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.
STUDY ON RADIATION DAMAGE ON A CRYOCOOLED CRYSTAL. REFINED PART 6: STRUCTURE AFTER A RADIATION DOSE OF 54*10E15 PHOTONSTUDY ON RADIATION DAMAGE ON A CRYOCOOLED CRYSTAL. REFINED PART 6: STRUCTURE AFTER A RADIATION DOSE OF 54*10E15 PHOTON
Full crystallographic information is available from OCA.
ReferenceReference
Structural changes in a cryo-cooled protein crystal owing to radiation damage., Burmeister WP, Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):328-41. PMID:10713520
