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| [[Image:1dw3.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1dw3| PDB=1dw3 | SCENE= }} | | {{STRUCTURE_1dw3| PDB=1dw3 | SCENE= }} |
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| '''STRUCTURE OF A REDUCED OXYGEN BINDING CYTOCHROME C'''
| | ===STRUCTURE OF A REDUCED OXYGEN BINDING CYTOCHROME C=== |
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| ==Overview==
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| The photosynthetic bacterium Rhodobacter sphaeroides produces a heme protein (SHP), which is an unusual c-type cytochrome capable of transiently binding oxygen during autooxidation. Similar proteins have not only been observed in other photosynthetic bacteria but also in the obligate methylotroph Methylophilus methylotrophus and the metal reducing bacterium Shewanella putrefaciens. A three-dimensional structure of SHP was derived using the multiple isomorphous replacement phasing method. Besides a model for the oxidized state (to 1.82 A resolution), models for the reduced state (2.1 A resolution), the oxidized molecule liganded with cyanide (1. 90 A resolution), and the reduced molecule liganded with nitric oxide (2.20 A resolution) could be derived. The SHP structure represents a new variation of the class I cytochrome c fold. The oxidized state reveals a novel sixth heme ligand, Asn(88), which moves away from the iron upon reduction or when small molecules bind. The distal side of the heme has a striking resemblance to other heme proteins that bind gaseous compounds. In SHP the liberated amide group of Asn(88) stabilizes solvent-shielded ligands through a hydrogen bond. | | The line below this paragraph, {{ABSTRACT_PUBMED_10821858}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10821858 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10821858}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Disulfide bridge]] | | [[Category: Disulfide bridge]] |
| [[Category: Reduced]] | | [[Category: Reduced]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:21:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:42:26 2008'' |