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==== | ==Crystal Structure of a Self-Assembling DNA Crystal Scaffold with Rhombohedral Symmetry== | ||
<StructureSection load='6wjk' size='340' side='right'caption='[[6wjk]]' scene=''> | <StructureSection load='6wjk' size='340' side='right'caption='[[6wjk]], [[Resolution|resolution]] 4.51Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[6wjk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WJK FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjk OCA], [https://pdbe.org/6wjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wjk RCSB], [https://www.ebi.ac.uk/pdbsum/6wjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjk ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.514Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjk OCA], [https://pdbe.org/6wjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wjk RCSB], [https://www.ebi.ac.uk/pdbsum/6wjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjk ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 A) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 A) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 A resolution by molecular replacement. | |||
A Self-Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High-Resolution Structural Detail.,Simmons CR, MacCulloch T, Zhang F, Liu Y, Stephanopoulos N, Yan H Angew Chem Int Ed Engl. 2020 Oct 12;59(42):18619-18626. doi: , 10.1002/anie.202005505. Epub 2020 Aug 17. PMID:32533629<ref>PMID:32533629</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6wjk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: MacCulloch T]] | |||
[[Category: Simmons CR]] | |||
[[Category: Stephanopoulos N]] | |||
[[Category: Yan H]] | |||
Latest revision as of 17:27, 18 October 2023
Crystal Structure of a Self-Assembling DNA Crystal Scaffold with Rhombohedral SymmetryCrystal Structure of a Self-Assembling DNA Crystal Scaffold with Rhombohedral Symmetry
Structural highlights
Publication Abstract from PubMedDNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 A) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 A) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 A resolution by molecular replacement. A Self-Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High-Resolution Structural Detail.,Simmons CR, MacCulloch T, Zhang F, Liu Y, Stephanopoulos N, Yan H Angew Chem Int Ed Engl. 2020 Oct 12;59(42):18619-18626. doi: , 10.1002/anie.202005505. Epub 2020 Aug 17. PMID:32533629[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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