7d59: Difference between revisions
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==== | ==cryo-EM structure of human RNA polymerase III in apo state== | ||
<StructureSection load='7d59' size='340' side='right'caption='[[7d59]]' scene=''> | <StructureSection load='7d59' size='340' side='right'caption='[[7d59]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7d59]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D59 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d59 OCA], [https://pdbe.org/7d59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d59 RCSB], [https://www.ebi.ac.uk/pdbsum/7d59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d59 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d59 OCA], [https://pdbe.org/7d59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d59 RCSB], [https://www.ebi.ac.uk/pdbsum/7d59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d59 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Disease == | |||
[https://www.uniprot.org/uniprot/RPC1_HUMAN RPC1_HUMAN] Wiedemann-Rautenstrauch syndrome;Hypomyelination-cerebellar atrophy-hypoplasia of the corpus callosum syndrome;Hypomyelinating leukodystrophy-ataxia-hypodontia-hypomyelination syndrome;Odontoleukodystrophy;Tremor-ataxia-central hypomyelination syndrome;Hypomyelination-hypogonadotropic hypogonadism-hypodontia syndrome. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RPC1_HUMAN RPC1_HUMAN] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.<ref>PMID:19609254</ref> <ref>PMID:19631370</ref> | |||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Lan P]] | ||
[[Category: Lei M]] | |||
[[Category: Wan F]] | |||
[[Category: Wang Q]] | |||
[[Category: Wu J]] | |||
Latest revision as of 13:52, 27 March 2024
cryo-EM structure of human RNA polymerase III in apo statecryo-EM structure of human RNA polymerase III in apo state
Structural highlights
DiseaseRPC1_HUMAN Wiedemann-Rautenstrauch syndrome;Hypomyelination-cerebellar atrophy-hypoplasia of the corpus callosum syndrome;Hypomyelinating leukodystrophy-ataxia-hypodontia-hypomyelination syndrome;Odontoleukodystrophy;Tremor-ataxia-central hypomyelination syndrome;Hypomyelination-hypogonadotropic hypogonadism-hypodontia syndrome. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. FunctionRPC1_HUMAN DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.[1] [2] See AlsoReferences
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