5y2h: Difference between revisions

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<StructureSection load='5y2h' size='340' side='right'caption='[[5y2h]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='5y2h' size='340' side='right'caption='[[5y2h]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5y2h]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_rotavirus_g10 Bovine rotavirus g10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y2H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y2H FirstGlance]. <br>
<table><tr><td colspan='2'>[[5y2h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_rotavirus_G10 Bovine rotavirus G10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y2H FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y2h OCA], [http://pdbe.org/5y2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y2h RCSB], [http://www.ebi.ac.uk/pdbsum/5y2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y2h ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y2h OCA], [https://pdbe.org/5y2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y2h RCSB], [https://www.ebi.ac.uk/pdbsum/5y2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y2h ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q6QT01_9VIRU Q6QT01_9VIRU]
Rotavirus nonstructural protein 4, the first viral enterotoxin to be identified, is a multidomain, multifunctional glycoprotein. Earlier, we reported a Ca(2+)-bound coiled-coil tetrameric structure of the diarrhea-inducing region of NSP4 from the rotavirus strains SA11 and I321 and a Ca(2+)-free pentameric structure from the rotavirus strain ST3, all with a parallel arrangement of alpha-helices. pH was found to determine the oligomeric state: a basic pH favoured a tetramer, whereas an acidic pH favoured a pentamer. Here, we report two novel forms of the coiled-coil region of NSP4 from the bovine rotavirus strains MF66 and NCDV. These crystallized at acidic pH, forming antiparallel coiled-coil tetrameric structures without any bound Ca(2+) ion. Structural and mutational studies of the coiled-coil regions of NSP4 revealed that the nature of the residue at position 131 (Tyr/His) plays an important role in the observed structural diversity.
 
New tetrameric forms of the rotavirus NSP4 with antiparallel helices.,Kumar S, Ramappa R, Pamidimukkala K, Rao CD, Suguna K Arch Virol. 2018 Feb 17. pii: 10.1007/s00705-018-3753-6. doi:, 10.1007/s00705-018-3753-6. PMID:29455326<ref>PMID:29455326</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5y2h" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bovine rotavirus g10]]
[[Category: Bovine rotavirus G10]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Kumar, S]]
[[Category: Kumar S]]
[[Category: Suguna, K]]
[[Category: Suguna K]]
[[Category: Antiparallel tetramer nsp4 rotavirus coiled-coil mf66]]
[[Category: Viral protein]]

Latest revision as of 13:21, 27 March 2024

Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain MF66Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain MF66

Structural highlights

5y2h is a 4 chain structure with sequence from Bovine rotavirus G10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q6QT01_9VIRU

See Also

5y2h, resolution 2.60Å

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