5use: Difference between revisions

Latest revision as of 14:06, 2 August 2023

5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant

Structural highlights

5use is a 3 chain structure with sequence from Alkalihalobacillus halodurans C-125 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.73Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNH1_HALH5 Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.^[1]

References

↑ Nowotny M, Gaidamakov SA, Crouch RJ, Yang W. Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell. 2005 Jul 1;121(7):1005-16. PMID:15989951 doi:http://dx.doi.org/10.1016/j.cell.2005.04.024

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OCA

[1] Nowotny M, Gaidamakov SA, Crouch RJ, Yang W. Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell. 2005 Jul 1;121(7):1005-16. PMID:15989951 doi:http://dx.doi.org/10.1016/j.cell.2005.04.024

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5use' size='340' side='right'caption='[[5use]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5use]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5USE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5USE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5use]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_halodurans_C-125 Alkalihalobacillus halodurans C-125] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5USE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5USE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=T5S:'>T5S</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=T5S:2-deoxy-5-(methylselanyl)uridine+5-phosphate'>T5S</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5us2|5us2]], [[5usa|5usa]], [[5usg|5usg]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5use FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5use OCA], [https://pdbe.org/5use PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5use RCSB], [https://www.ebi.ac.uk/pdbsum/5use PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5use ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnhA, BH0863 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272558 BACHD])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5use FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5use OCA], [http://pdbe.org/5use PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5use RCSB], [http://www.ebi.ac.uk/pdbsum/5use PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5use ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RNH1_BACHD RNH1_BACHD]] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
+[https://www.uniprot.org/uniprot/RNH1_HALH5 RNH1_HALH5] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.<ref>PMID:15989951</ref>
 ==See Also==
 *[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
+== References ==
+<references/>
 __TOC__
 </StructureSection>
-[[Category: Bachd]]
+[[Category: Alkalihalobacillus halodurans C-125]]
 [[Category: Large Structures]]
-[[Category: Ribonuclease H]]
+[[Category: Synthetic construct]]
-[[Category: Fang, Z]]
+[[Category: Fang Z]]
-[[Category: Huang, Z]]
+[[Category: Huang Z]]
-[[Category: Qin, L]]
+[[Category: Qin L]]
-[[Category: Hydrolase - rna - dna complex]]

5use: Difference between revisions

Latest revision as of 14:06, 2 August 2023

5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant

Structural highlights

Function

See Also

References

Contents

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5use: Difference between revisions

Latest revision as of 14:06, 2 August 2023

5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant

Structural highlights

Function

See Also

References

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