6t7s: Difference between revisions
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==MexB structure solved by cryo-EM in nanodisc in absence of its protein partners== | ==MexB structure solved by cryo-EM in nanodisc in absence of its protein partners== | ||
<StructureSection load='6t7s' size='340' side='right'caption='[[6t7s]]' scene=''> | <StructureSection load='6t7s' size='340' side='right'caption='[[6t7s]], [[Resolution|resolution]] 4.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T7S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T7S FirstGlance]. <br> | <table><tr><td colspan='2'>[[6t7s]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T7S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6T7S FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t7s OCA], [http://pdbe.org/6t7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t7s RCSB], [http://www.ebi.ac.uk/pdbsum/6t7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t7s ProSAT]</span></td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ta5|6ta5]], [[6ta6|6ta6]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mexB, mexB_1, C0044_03030, DZ934_11475, E4V10_16065, IPC3_09885, IPC669_14600, PAERUG_E15_London_28_01_14_02845, PAMH19_0483, RW109_RW109_01030 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6t7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t7s OCA], [http://pdbe.org/6t7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t7s RCSB], [http://www.ebi.ac.uk/pdbsum/6t7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t7s ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The tripartite multidrug efflux system MexAB-OprM is a major actor in Pseudomonas aeruginosa antibiotic resistance by exporting a large variety of antimicrobial compounds. Crystal structures of MexB and of its Escherichia coli homolog AcrB had revealed asymmetric trimers depicting a directional drug pathway by a conformational interconversion (from Loose and Tight binding pockets to Open gate (LTO) for drug exit). It remains unclear how MexB acquires its LTO form. Here by performing functional and cryo-EM structural investigations of MexB at various stages of the assembly process, we unveil that MexB inserted in lipid membrane is not set for active transport because it displays an inactive LTC form with a Closed exit gate. In the tripartite complex, OprM and MexA form a corset-like platform that converts MexB into the active form. Our findings shed new light on the resistance nodulation cell division (RND) cognate partners which act as allosteric factors eliciting the functional drug extrusion. | |||
Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.,Glavier M, Puvanendran D, Salvador D, Decossas M, Phan G, Garnier C, Frezza E, Cece Q, Schoehn G, Picard M, Taveau JC, Daury L, Broutin I, Lambert O Nat Commun. 2020 Oct 2;11(1):4948. doi: 10.1038/s41467-020-18770-5. PMID:33009415<ref>PMID:33009415</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6t7s" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Broutin I]] | [[Category: Broutin, I]] | ||
[[Category: Daury L]] | [[Category: Daury, L]] | ||
[[Category: Glavier M]] | [[Category: Glavier, M]] | ||
[[Category: Lambert O]] | [[Category: Lambert, O]] | ||
[[Category: Phan G]] | [[Category: Phan, G]] | ||
[[Category: Schoehn G]] | [[Category: Schoehn, G]] | ||
[[Category: Taveau | [[Category: Taveau, J C]] | ||
[[Category: Antimicrobial protein]] | |||
[[Category: Bacterial resistance]] | |||
[[Category: Efflux]] | |||
[[Category: Proton motive force]] | |||
[[Category: Transporter]] | |||