6m4e: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of a GH1 beta-glucosidase from Hamamotoa singularis== | ==Crystal structure of a GH1 beta-glucosidase from Hamamotoa singularis== | ||
<StructureSection load='6m4e' size='340' side='right'caption='[[6m4e]]' scene=''> | <StructureSection load='6m4e' size='340' side='right'caption='[[6m4e]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M4E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M4E FirstGlance]. <br> | <table><tr><td colspan='2'>[[6m4e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24193 Atcc 24193]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M4E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M4E FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m4e OCA], [http://pdbe.org/6m4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m4e RCSB], [http://www.ebi.ac.uk/pdbsum/6m4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m4e ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bglA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5445 ATCC 24193])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m4e OCA], [http://pdbe.org/6m4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m4e RCSB], [http://www.ebi.ac.uk/pdbsum/6m4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m4e ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A GH1 beta-glucosidase from the fungus Hamamotoa singularis (HsBglA) has high transgalactosylation activity and efficiently converts lactose to galactooligosaccharides. Consequently, HsBglA is among the most widely used enzymes for industrial galactooligosaccharide production. Here, we present the first crystal structures of HsBglA with and without 4'-galactosyllactose, a tri-galactooligosaccharide, at 3.0 and 2.1 A resolutions, respectively. These structures reveal details of the structural elements that define the catalytic activity and substrate binding of HsBglA, and provide a possible interpretation for its high catalytic potency for transgalactosylation reaction. | |||
Crystal structure of a GH1 beta-glucosidase from Hamamotoa singularis.,Uehara R, Iwamoto R, Aoki S, Yoshizawa T, Takano K, Matsumura H, Tanaka SI Protein Sci. 2020 Jul 26. doi: 10.1002/pro.3916. PMID:32713015<ref>PMID:32713015</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6m4e" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 24193]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Aoki S]] | [[Category: Aoki, S]] | ||
[[Category: Iwamoto R]] | [[Category: Iwamoto, R]] | ||
[[Category: Matsumura H]] | [[Category: Matsumura, H]] | ||
[[Category: Takano K]] | [[Category: Takano, K]] | ||
[[Category: Tanaka S | [[Category: Tanaka, S i]] | ||
[[Category: Uehara R]] | [[Category: Uehara, R]] | ||
[[Category: Yoshizawa T]] | [[Category: Yoshizawa, T]] | ||
[[Category: Beta-galactosidase]] | |||
[[Category: Beta-glucosidase]] | |||
[[Category: Galactooligosaccharide]] | |||
[[Category: Hamamotoa singulari]] | |||
[[Category: Hydrolase]] | |||
[[Category: Structural protein]] | |||
[[Category: Transgalactosylation]] | |||