3dlb: Difference between revisions

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 <StructureSection load='3dlb' size='340' side='right'caption='[[3dlb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3dlb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DLB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3DLB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3dlb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DLB FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dlh|3dlh]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3dlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dlb OCA], [http://pdbe.org/3dlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dlb RCSB], [http://www.ebi.ac.uk/pdbsum/3dlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dlb ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dlb OCA], [https://pdbe.org/3dlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dlb RCSB], [https://www.ebi.ac.uk/pdbsum/3dlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dlb ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q746M7_THET2 Q746M7_THET2]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
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 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dlb ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-The slicer activity of the RNA-induced silencing complex is associated with argonaute, the RNase H-like PIWI domain of which catalyses guide-strand-mediated sequence-specific cleavage of target messenger RNA. Here we report on the crystal structure of Thermus thermophilus argonaute bound to a 5'-phosphorylated 21-base DNA guide strand, thereby identifying the nucleic-acid-binding channel positioned between the PAZ- and PIWI-containing lobes, as well as the pivot-like conformational changes associated with complex formation. The bound guide strand is anchored at both of its ends, with the solvent-exposed Watson-Crick edges of stacked bases 2 to 6 positioned for nucleation with the mRNA target, whereas two critically positioned arginines lock bases 10 and 11 at the cleavage site into an unanticipated orthogonal alignment. Biochemical studies indicate that key amino acid residues at the active site and those lining the 5'-phosphate-binding pocket made up of the Mid domain are critical for cleavage activity, whereas alterations of residues lining the 2-nucleotide 3'-end-binding pocket made up of the PAZ domain show little effect.
-Structure of the guide-strand-containing argonaute silencing complex.,Wang Y, Sheng G, Juranek S, Tuschl T, Patel DJ Nature. 2008 Nov 13;456(7219):209-13. Epub 2008 Aug 27. PMID:18754009<ref>PMID:18754009</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3dlb" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Argonaute 3D structures|Argonaute 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
 [[Category: Large Structures]]
-[[Category: Patel, D J]]
+[[Category: Thermus thermophilus]]
-[[Category: Sheng, G]]
+[[Category: Patel DJ]]
-[[Category: Wang, Y]]
+[[Category: Sheng G]]
-[[Category: Argonaute]]
+[[Category: Wang Y]]
-[[Category: Nucleic acid binding protein-dna complex]]
-[[Category: Plasmid]]
-[[Category: Protein-dna complex]]