1m4p: Difference between revisions
New page: left|200px<br /> <applet load="1m4p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4p" /> '''Structure of the Tsg101 UEV domain in compl... |
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==Overview== | ==Overview== | ||
The structural proteins of HIV and Ebola display PTAP peptide motifs, (termed 'late domains') that recruit the human protein Tsg101 to, facilitate virus budding. Here we present the solution structure of the, UEV (ubiquitin E2 variant) binding domain of Tsg101 in complex with a PTAP, peptide that spans the late domain of HIV-1 p6(Gag). The UEV domain of, Tsg101 resembles E2 ubiquitin-conjugating enzymes, and the PTAP peptide, binds in a bifurcated groove above the vestigial enzyme active site. Each, PTAP residue makes important contacts, and the Ala 9-Pro 10 dipeptide, binds in a deep pocket of the UEV domain that resembles the X-Pro binding, pockets of SH3 and WW domains. The structure reveals the molecular basis, of HIV PTAP late domain function and represents an attractive starting, point for the design of novel inhibitors of virus budding. | The structural proteins of HIV and Ebola display PTAP peptide motifs, (termed 'late domains') that recruit the human protein Tsg101 to, facilitate virus budding. Here we present the solution structure of the, UEV (ubiquitin E2 variant) binding domain of Tsg101 in complex with a PTAP, peptide that spans the late domain of HIV-1 p6(Gag). The UEV domain of, Tsg101 resembles E2 ubiquitin-conjugating enzymes, and the PTAP peptide, binds in a bifurcated groove above the vestigial enzyme active site. Each, PTAP residue makes important contacts, and the Ala 9-Pro 10 dipeptide, binds in a deep pocket of the UEV domain that resembles the X-Pro binding, pockets of SH3 and WW domains. The structure reveals the molecular basis, of HIV PTAP late domain function and represents an attractive starting, point for the design of novel inhibitors of virus budding. | ||
==Disease== | |||
Known disease associated with this structure: Breast cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601387 601387]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: virus budding]] | [[Category: virus budding]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:07:21 2007'' | ||
