6y98: Difference between revisions
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<StructureSection load='6y98' size='340' side='right'caption='[[6y98]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='6y98' size='340' side='right'caption='[[6y98]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6y98]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6y98]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_glabrata_CBS_138 Candida glabrata CBS 138]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y98 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>< | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y98 OCA], [https://pdbe.org/6y98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y98 RCSB], [https://www.ebi.ac.uk/pdbsum/6y98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y98 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B4UMX2_CANGA B4UMX2_CANGA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Diderrich | [[Category: Diderrich R]] | ||
[[Category: Essen | [[Category: Essen L-O]] | ||
[[Category: Friederichs | [[Category: Friederichs S]] | ||
[[Category: Hoffmann | [[Category: Hoffmann D]] | ||
[[Category: Kock | [[Category: Kock M]] | ||
[[Category: Moesch | [[Category: Moesch H-U]] | ||
[[Category: Reithofer | [[Category: Reithofer V]] | ||
Revision as of 16:22, 24 January 2024
Crystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactoseCrystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactose
Structural highlights
FunctionPublication Abstract from PubMedFor host-cell interaction, the human fungal pathogen Candida glabrata harbors a large family of more than 20 cell wall attached epithelial adhesins (Epas). Epa family members are lectins with binding pockets containing several conserved and variable structural hot spots, which were implicated in mediating functional diversity. In this study, we have performed an elaborate structure-based mutational analysis of numerous Epa paralogs, in order to generally determine the role of diverse structural hot spots in conferring host cell binding and ligand binding specificity. Our study reveals that several conserved structural motifs contribute to efficient host cell binding. Moreover, our directed motif exchange experiments reveal that the variable loop CBL2 is key for programming ligand binding specificity, albeit with limited predictability. In contrast, we find that the variable loop L1 affects host cell binding without significantly influencing specificity of ligand binding. Our data strongly suggest, that variation of numerous structural hot spots in the ligand binding pocket of Epa proteins is a main driver of their functional diversification and evolution. Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.,Hoffmann D, Diderrich R, Reithofer V, Friederichs S, Kock M, Essen LO, Mosch HU J Biol Chem. 2020 Jul 15. pii: RA120.013968. doi: 10.1074/jbc.RA120.013968. PMID:32669365[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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