2yw2: Difference between revisions
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<StructureSection load='2yw2' size='340' side='right'caption='[[2yw2]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='2yw2' size='340' side='right'caption='[[2yw2]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2yw2]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2yw2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YW2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yw2 OCA], [https://pdbe.org/2yw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yw2 RCSB], [https://www.ebi.ac.uk/pdbsum/2yw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yw2 ProSAT], [https://www.topsan.org/Proteins/RSGI/2yw2 TOPSAN]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PUR2_AQUAE PUR2_AQUAE] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Aquifex aeolicus | [[Category: Aquifex aeolicus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Baba S]] | |||
[[Category: Baba | [[Category: Kanagawa M]] | ||
[[Category: Kanagawa | [[Category: Kawai G]] | ||
[[Category: Kawai | [[Category: Kuramitsu S]] | ||
[[Category: Kuramitsu | [[Category: Sampei G]] | ||
[[Category: Yokoyama S]] | |||
[[Category: Sampei | |||
[[Category: Yokoyama | |||
Revision as of 12:06, 25 October 2023
Crystal structure of GAR synthetase from Aquifex aeolicus in complex with ATPCrystal structure of GAR synthetase from Aquifex aeolicus in complex with ATP
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedGlycinamide ribonucleotide synthetase (GAR-syn, PurD) catalyses the second reaction of the purine biosynthetic pathway; the conversion of phosphoribosylamine, glycine and ATP to glycinamide ribonucleotide (GAR), ADP and Pi. In the present study, crystal structures of GAR-syn's from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus were determined in apo forms. Crystal structures in ligand-bound forms were also determined for G. kaustophilus and A. aeolicus proteins. In general, overall structures of GAR-syn's are similar to each other. However, the orientations of the B domains are varied among GAR-syn's and the MD simulation suggested the mobility of the B domain. Furthermore, it was demonstrated that the B loop in the B domain fixes the position of the beta- and gamma- phosphate groups of the bound ATP. The structures of GAR-syn's and the bound ligands were compared with each other in detail, and structures of GAR-syn's with full ligands, as well as the possible reaction mechanism, were proposed. Crystal structures of glycinamide ribonucleotide synthetase, PurD, from thermophilic eubacteria.,Sampei G, Baba S, Kanagawa M, Yanai H, Ishii T, Kawai H, Fukai Y, Ebihara A, Nakagawa N, Kawai G J Biochem. 2010 Oct;148(4):429-38. Epub 2010 Aug 16. PMID:20716513[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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