6te9: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==== | ==Neck of native GTA particle computed with C6 symmetry== | ||
<StructureSection load='6te9' size='340' side='right'caption='[[6te9]]' scene=''> | <StructureSection load='6te9' size='340' side='right'caption='[[6te9]], [[Resolution|resolution]] 3.58Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6te9]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TE9 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.58Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6te9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6te9 OCA], [https://pdbe.org/6te9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6te9 RCSB], [https://www.ebi.ac.uk/pdbsum/6te9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6te9 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/D5ATZ4_RHOCB D5ATZ4_RHOCB] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. | |||
Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663<ref>PMID:32541663</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6te9" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Portal protein 3D structures|Portal protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Rhodobacter capsulatus]] | ||
[[Category: Bardy P]] | |||
[[Category: Beatty JT]] | |||
[[Category: Fuzik T]] | |||
[[Category: Hrebik D]] | |||
[[Category: Pantucek R]] | |||
[[Category: Plevka P]] | |||
Latest revision as of 13:17, 22 May 2024
Neck of native GTA particle computed with C6 symmetryNeck of native GTA particle computed with C6 symmetry
Structural highlights
FunctionPublication Abstract from PubMedAlphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||