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| <StructureSection load='6vms' size='340' side='right'caption='[[6vms]], [[Resolution|resolution]] 3.80Å' scene=''> | | <StructureSection load='6vms' size='340' side='right'caption='[[6vms]], [[Resolution|resolution]] 3.80Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6vms]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VMS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VMS FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VMS FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=08Y:BROMOERGOCRYPTINE'>08Y</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gnai1, Gnai-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), GNB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), GNG2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), DRD2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=08Y:BROMOERGOCRYPTINE'>08Y</scene></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vms OCA], [https://pdbe.org/6vms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vms RCSB], [https://www.ebi.ac.uk/pdbsum/6vms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vms ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vms OCA], [http://pdbe.org/6vms PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vms RCSB], [http://www.ebi.ac.uk/pdbsum/6vms PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vms ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
| |
| [[http://www.uniprot.org/uniprot/GNAI1_RAT GNAI1_RAT]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:16870394</ref> [[http://www.uniprot.org/uniprot/GBG2_HUMAN GBG2_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (By similarity). [[http://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
| |
| The D2 dopamine receptor (DRD2) is a therapeutic target for Parkinson's disease(1) and antipsychotic drugs(2). DRD2 is activated by the endogenous neurotransmitter dopamine and synthetic agonist drugs such as bromocriptine(3), leading to stimulation of Gi and inhibition of adenylyl cyclase. Here we used cryo-electron microscopy to elucidate the structure of an agonist-bound activated DRD2-Gi complex reconstituted into a phospholipid membrane. The extracellular ligand-binding site of DRD2 is remodelled in response to agonist binding, with conformational changes in extracellular loop 2, transmembrane domain 5 (TM5), TM6 and TM7, propagating to opening of the intracellular Gi-binding site. The DRD2-Gi structure represents, to our knowledge, the first experimental model of a G-protein-coupled receptor-G-protein complex embedded in a phospholipid bilayer, which serves as a benchmark to validate the interactions seen in previous detergent-bound structures. The structure also reveals interactions that are unique to the membrane-embedded complex, including helix 8 burial in the inner leaflet, ordered lysine and arginine side chains in the membrane interfacial regions, and lipid anchoring of the G protein in the membrane. Our model of the activated DRD2 will help to inform the design of subtype-selective DRD2 ligands for multiple human central nervous system disorders.
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| Structure of a D2 dopamine receptor-G-protein complex in a lipid membrane.,Yin J, Chen KM, Clark MJ, Hijazi M, Kumari P, Bai XC, Sunahara RK, Barth P, Rosenbaum DM Nature. 2020 Jun 11. pii: 10.1038/s41586-020-2379-5. doi:, 10.1038/s41586-020-2379-5. PMID:32528175<ref>PMID:32528175</ref>
| | ==See Also== |
| | | *[[Dopamine receptor 3D structures|Dopamine receptor 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| | *[[Transducin 3D structures|Transducin 3D structures]] |
| </div>
| |
| <div class="pdbe-citations 6vms" style="background-color:#fffaf0;"></div>
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| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Buffalo rat]]
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| [[Category: Human]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Lysozyme]]
| | [[Category: Bai X]] |
| [[Category: Bai, X]] | | [[Category: Barth P]] |
| [[Category: Barth, P]] | | [[Category: Chen KM]] |
| [[Category: Chen, K M]] | | [[Category: Clark MJ]] |
| [[Category: Clark, M J]] | | [[Category: Hijazi M]] |
| [[Category: Hijazi, M]] | | [[Category: Kumari P]] |
| [[Category: Kumari, P]] | | [[Category: Rosenbaum DM]] |
| [[Category: Rosenbaum, D M]] | | [[Category: Sunahara RK]] |
| [[Category: Sunahara, R K]] | | [[Category: Yin J]] |
| [[Category: Yin, J]] | |
| [[Category: Dopamine]]
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| [[Category: Dopamine receptor]]
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| [[Category: G protein]]
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| [[Category: Gpcr]]
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| [[Category: Parkinson's disease]]
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| [[Category: Signaling protein]]
| |